Probing structure and reaction dynamics of proteins using time-resolved resonance Raman spectroscopy

D Buhrke, P Hildebrandt - Chemical reviews, 2019 - ACS Publications
The mechanistic understanding of protein functions requires insight into the structural and
reaction dynamics. To elucidate these processes, a variety of experimental approaches are …

Tips and turns of bacteriophytochrome photoactivation

H Takala, P Edlund, JA Ihalainen… - Photochemical & …, 2020 - pubs.rsc.org
Phytochromes are ubiquitous photosensor proteins, which control the growth, reproduction
and movement in plants, fungi and bacteria. Phytochromes switch between two …

Time-resolved serial femtosecond crystallography at the European XFEL

S Pandey, R Bean, T Sato, I Poudyal, J Bielecki… - Nature …, 2020 - nature.com
Abstract The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces
femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition …

Protein control of photochemistry and transient intermediates in phytochromes

G Salvadori, V Macaluso, G Pellicci, L Cupellini… - Nature …, 2022 - nature.com
Phytochromes are ubiquitous photoreceptors responsible for sensing light in plants, fungi
and bacteria. Their photoactivation is initiated by the photoisomerization of the embedded …

Photoactivation mechanism, timing of protein secondary structure dynamics and carotenoid translocation in the orange carotenoid protein

PE Konold, IHM Van Stokkum… - Journal of the …, 2018 - ACS Publications
The orange carotenoid protein (OCP) is a two-domain photoactive protein that noncovalently
binds an echinenone (ECN) carotenoid and mediates photoprotection in cyanobacteria. In …

High-resolution crystal structures of transient intermediates in the phytochrome photocycle

M Carrillo, S Pandey, J Sanchez, M Noda, I Poudyal… - Structure, 2021 - cell.com
Phytochromes are red/far-red light photoreceptors in bacteria to plants, which elicit a variety
of important physiological responses. They display a reversible photocycle between the …

Correlating structural and photochemical heterogeneity in cyanobacteriochrome NpR6012g4

S Lim, Q Yu, SM Gottlieb, CW Chang… - Proceedings of the …, 2018 - National Acad Sciences
Phytochrome photoreceptors control plant growth, development, and the shade avoidance
response that limits crop yield in high-density agricultural plantings. Cyanobacteriochromes …

Chromophore–protein interplay during the phytochrome photocycle revealed by step-scan FTIR spectroscopy

JA Ihalainen, E Gustavsson, L Schroeder… - Journal of the …, 2018 - ACS Publications
Phytochrome proteins regulate many photoresponses of plants and microorganisms. Light
absorption causes isomerization of the biliverdin chromophore, which triggers a series of …

Reaction dynamics of the chimeric channelrhodopsin C1C2

Y Hontani, M Marazzi, K Stehfest, T Mathes… - Scientific Reports, 2017 - nature.com
Channelrhodopsin (ChR) is a key protein of the optogenetic toolkit. C1C2, a functional
chimeric protein of Chlamydomonas reinhardtii ChR1 and ChR2, is the only ChR whose …

Mechanistic investigations of green mEos4b reveal a dynamic long-lived dark state

E De Zitter, J Ridard, D Thédié, V Adam… - Journal of the …, 2020 - ACS Publications
Green-to-red photoconvertible fluorescent proteins (PCFPs) are key players in advanced
microscopy schemes such as photoactivated localization microscopy (PALM). Whereas …