Methods of inactivation of SARS-CoV-2 for downstream biological assays

EI Patterson, T Prince, ER Anderson… - The Journal of …, 2020 - academic.oup.com
The scientific community has responded to the coronavirus disease 2019 (COVID-19)
pandemic by rapidly undertaking research to find effective strategies to reduce the burden of …

The impact of thermal pasteurization on viral load and detectable live viruses in human milk and other matrices: a rapid review

MA Pitino, DL O'Connor, AJ McGeer… - Applied Physiology …, 2021 - cdnsciencepub.com
Holder pasteurization (62.5° C, 30 min) of human milk is thought to reduce the risk of
transmitting viruses to an infant. Some viruses may be secreted into milk–others may be …

Heat inactivation of clinical COVID-19 samples on an industrial scale for low risk and efficient high-throughput qRT-PCR diagnostic testing

O Delpuech, JA Douthwaite, T Hill, D Niranjan… - Scientific Reports, 2022 - nature.com
We report the development of a large scale process for heat inactivation of clinical COVID-
19 samples prior to laboratory processing for detection of SARS-CoV-2 by RT-qPCR. With …

The impact of thermal pasteurization on viral load in human milk and other matrices: A rapid review

MA Pitino, DL O'Connor, AJ McGeer, S Unger - MedRxiv, 2020 - medrxiv.org
Abstract Holder pasteurization (62.5° C, 30 min) of human milk (HM) is thought to reduce the
risk of transmitting viruses to an infant. Some viruses may be secreted into milk–others may …

Use of guanidine thiocyanate-based nucleic acid extraction buffers to inactivate poliovirus in potentially infectious materials

MJ Honeywood, S Jeffries-Miles, K Wong… - Journal of virological …, 2021 - Elsevier
The efforts of the Global Poliovirus Eradication Initiative (GPEI) have brought about the near
elimination of poliovirus worldwide. The World Health Organization has issued guidelines …

Inactivation of SARS-CoV-2 on surfaces and in solution with Virusend (TX-10), a novel disinfectant

ER Anderson, GL Hughes… - Access …, 2021 - microbiologyresearch.org
Until an effective vaccine against SARS-CoV-2 is available on a widespread scale, the
control of the COVID-19 pandemic is reliant upon effective pandemic control measures. The …

[HTML][HTML] Inactivation Validation of Ebola, Marburg, and Lassa Viruses in AVL and Ethanol-Treated Viral Cultures

T Cutts, A Leung, L Banadyga, J Krishnan - Viruses, 2024 - mdpi.com
High-consequence pathogens such as the Ebola, Marburg, and Lassa viruses are handled
in maximum-containment biosafety level 4 (BSL-4) laboratories. Genetic material is often …

Comprehensive Assessment of Inactivation Methods for Madariaga Virus

RM Boytz, K Keita, JB Pawlak, M Laurent-Rolle - Viruses, 2024 - mdpi.com
The Eastern Equine Encephalitis Virus (EEEV) is an emerging public health threat, with the
number of reported cases in the US increasing in recent years. EEEV is a BSL3 pathogen …

Measuring alphavirus fidelity using non-infectious virus particles

EI Patterson, K Khanipov, DM Swetnam, S Walsdorf… - Viruses, 2020 - mdpi.com
Mutations are incorporated into the genomes of RNA viruses at an optimal frequency and
altering this precise frequency has been proposed as a strategy to create live-attenuated …

A roadmap for developing Venezuelan equine encephalitis virus (VEEV) vaccines: Lessons from the past, strategies for the future

L Han, S Song, H Feng, J Ma, W Wei, F Si - International Journal of …, 2023 - Elsevier
Venezuelan equine encephalitis (VEE) is a zoonotic infectious disease caused by the
Venezuelan equine encephalitis virus (VEEV), which can lead to severe central nervous …