Frontiers in artificial intelligence‐directed light‐sheet microscopy for uncovering biological phenomena and multiorgan imaging

E Zhu, YR Li, S Margolis, J Wang, K Wang, Y Zhang… - View, 2024 - Wiley Online Library
Light‐sheet fluorescence microscopy (LSFM) introduces fast scanning of biological
phenomena with deep photon penetration and minimal phototoxicity. This advancement …

Near-Infrared Emissive π-Conjugated Oligomer Nanoparticles for Three-and Four-Photon Deep-Brain Microscopic Imaging Beyond 1700 nm Excitation

L Kan, Y Zhang, Y Luo, Y Wei, J Zhong, Y Gao, Y Liu… - ACS …, 2024 - ACS Publications
High-resolution visualization of the deep brain is still a challenging and very significant
issue. Multiphoton microscopy (MPM) holds great promise for high-spatiotemporal deep …

SELMA3D challenge: Self-supervised learning for 3D light-sheet microscopy image segmentation

Y Chen, R Al-Maskari, I Horvath, M Ali, L Höher… - arXiv preprint arXiv …, 2025 - arxiv.org
Recent innovations in light sheet microscopy, paired with developments in tissue clearing
techniques, enable the 3D imaging of large mammalian tissues with cellular resolution …

In situ isotropic 3D imaging of vasculature perfusion specimens using x‐ray microscopic dual‐energy CT

S Handschuh, U Reichart, S Kummer… - Journal of …, 2025 - Wiley Online Library
Ex vivo x‐ray angiography provides high‐resolution, three‐dimensional information on
vascular phenotypes down to the level of capillaries. Sample preparation for ex vivo …

骨科学领域组织透明化技术的应用与展望

高阳, 高原, 马炳江, 岳辉, 石永新 - 中国组织工程研究, 2025 - cjter.com
摘要背景: 骨组织作为高度矿化且结构复杂的结缔组织, 在维持生物体形态和功能中扮演关键
角色, 传统的成像技术难以满足对其深入观察的需求. 组织透明化技术的出现使得研究者能够 …

[HTML][HTML] Protocol for three-dimensional whole-mount imaging of the vascular network in the intestinal muscle

S Schrenk, LJ Bischoff, E Boscolo - STAR protocols, 2024 - Elsevier
Summary Three-dimensional (3D) imaging of vascular networks is essential for the
investigation of vascular patterning and organization. Here, we present a step-by-step …

Applications and prospects of tissue clearing technology in field of orthopedics

Y Gao, Y Gao, B Ma, H Yue, Y Shi - Chinese Journal of Tissue …, 2025 - cjter.com
BACKGROUND: Bone tissue, as a highly mineralized and structurally complex connective
tissue, plays a pivotal role in maintaining the form and function of living organisms …