Bacterial genome reductions: Tools, applications, and challenges

N LeBlanc, TC Charles - Frontiers in Genome Editing, 2022 - frontiersin.org
Bacterial cells are widely used to produce value-added products due to their versatility, ease
of manipulation, and the abundance of genome engineering tools. However, the efficiency of …

Beware of mycoplasma anti-immunoglobulin strategies

Y Arfi, C Lartigue, P Sirand-Pugnet, A Blanchard - Mbio, 2021 - Am Soc Microbiol
Mycoplasmas are small, genome-reduced bacteria. They are obligate parasites that can be
found in a wide range of host species, including the majority of livestock animals and …

Transcriptomics and proteomics revealed the psychrotolerant and antibiotic-resistant mechanisms of strain Pseudomonas psychrophila RNC-1 capable of assimilatory …

Y Zhao, H Min, K Luo, R Zhang, Q Chen… - Science of The Total …, 2022 - Elsevier
Aerobic denitrification has been proved to be profoundly affected by temperature and
antibiotics, but little is known about how aerobic denitrifiers respond to temperature and …

A genetic toolkit and gene switches to limit Mycoplasma growth for biosafety applications

A Broto, E Gaspari, S Miravet-Verde… - Nature …, 2022 - nature.com
Mycoplasmas have exceptionally streamlined genomes and are strongly adapted to their
many hosts, which provide them with essential nutrients. Owing to their relative genomic …

The metabolic process of methane production by combined fermentation of coal and corn straw

H Guo, M Zhang, Z Chen, Y Shen, J Lv, X Xu… - Bioresource …, 2021 - Elsevier
The anaerobic degradation of coal combined with straw biomass can promote the methane
production. The biogas production potential and metabolic pathway were explored via the …

Response of the aerobic denitrifying phosphorus accumulating bacteria Pseudomonas psychrophila HA-2 to low temperature and zinc oxide nanoparticles stress

K Luo, L Chen, L Du, Y Zhao, Q Chen - Bioresource Technology, 2022 - Elsevier
Performance and molecular changes of an aerobic denitrifying phosphorus accumulating
bacteria Pseudomonas psychrophila HA-2 have been investigated under different …

Genes required for survival and proliferation of Mycoplasma bovis in association with host cells

CK Premachandre, PK Vaz, S Sharma… - Applied and …, 2024 - Am Soc Microbiol
Mycoplasma bovis is an important emerging pathogen of cattle and bison, but our
understanding of the genetic basis of its interactions with its host is limited. The aim of this …

[HTML][HTML] Engineering Mycoplasma pneumoniae to bypass the association with Guillain-Barré syndrome

A Broto, C Piñero-Lambea, C Segura-Morales… - Microbes and …, 2024 - Elsevier
A non-pathogenic Mycoplasma pneumoniae-based chassis is leading the development of
live biotherapeutic products (LBPs) for respiratory diseases. However, reports connecting …

LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions

D Shaw, S Miravet‐Verde… - Microbial …, 2021 - Wiley Online Library
The removal of unwanted genetic material is a key aspect in many synthetic biology efforts
and often requires preliminary knowledge of which genomic regions are dispensable …

MycoWiki: Functional annotation of the minimal model organism Mycoplasma pneumoniae

C Elfmann, B Zhu, T Pedreira, B Hoßbach… - Frontiers in …, 2022 - frontiersin.org
The human pathogen Mycoplasma pneumoniae is viable independently from host cells or
organisms, despite its strongly reduced genome with only about 700 protein-coding genes …