For many years, DNA gyrase was thought to be responsible both for unlinking replicated
daughter chromosomes and for controlling negative superhelical tension in bacterial DNA …

The various problems of disentangling DNA strands or duplexes in a cell are all rooted in the
double-helical structure of DNA. Three distinct subfamilies of enzymes, known as the DNA …

DNA gyrase and topoisomerase IV are the two type II topoisomerases present in bacteria.
Though clearly related, based on amino acid sequence similarity, they each play crucial, but …

The evolutionary relationships of proteobacteria, which comprise the largest and
phenotypically most diverse division among prokaryotes, are examined based on the …

INTRODUCTION The faithful replication and segregation of chromosomes as part of the cell
division cycle requires that topological changes be imposed upon cellular DNA …

Two DNA topoisomerases control the level of negative supercoiling in bacterial cells. DNA
gyrase introduces supercoils, and DNA topoisomerase I prevents super‐coiling from …

A 4.6 kb Staphylococcus aureus DNA fragment containing DNA gyrase‐like genes (grlA and
grlB) was cloned and sequenced. The proteins GrlA and GrlB exhibit more than 30% identity …

For a cell to complete DNA replication, every link between the Watson-Crick strands must be
removed by topoisomerases. Previously, we reported that the inhibition of topoisomerase IV …

Mutants in bacterial topoisomerase(topo) IV are deficient in chromosomal partitioning. To
investigate the basis of this phenotype, we examined plasmid DNA topology in conditionally …

Fifteen strains of Escherichia coli with MICs of ciprofloxacin (CIP) between 0.015 and 256
micrograms/ml were examined for the presence of mutations in the quinolone resistance …