Over the last five years, the number of clinical trials involving AAV (adeno-associated virus)
and lentiviral vectors continue to increase by about 150 trials each year. For continued …

Synthetic biology is a logical extension of what has been called recombinant DNA (rDNA)
technology or genetic engineering since the 1970s. As rDNA technology has been the driver …

BACKGROUND Recessive dystrophic epidermolysis bullosa (RDEB) is a severe form of skin
fragility disorder due to mutations in COL7A1 encoding basement membrane type VII …

Epigenetic silencing of transgene expression represents a major obstacle for the efficient
genetic modification of multipotent and pluripotent stem cells. We and others have …

The isolation of stably transfected cell lines suitable for the manufacture of biotherapeutic
protein products can be an arduous process relying on the identification of a high …

Correction of patient-specific induced pluripotent stem cells (iPSC) upon gene delivery
through retroviral vectors offers new treatment perspectives for monogenetic diseases. Gene …

Induced pluripotent stem cell (iPSC) reprogramming requires sustained expression of
multiple reprogramming factors for a limited period of time (10–30 days). Conventional iPSC …

Abstract Ubiquitous Chromatin-opening Elements (UCOEs) are defined by their ability to
consistently confer stable, site of integration-independent transgene expression that is …

Highlights•Cell lineage underlies the developmental steps taken to acquire cell
identity.•Genetic labeling allows lineage tracing with cell type and temporal …

Bone is a highly dynamic and specialized tissue, capable of regenerating itself
spontaneously when afflicted by minor injuries. Nevertheless, when major lesions occur, it …