Impacts of climate change like global warming, drought, flooding, and other extreme events
are posing severe challenges to global crop production. Contribution of Brassica napus …

This review examines how real-time PCR can be used to determine copy number and
zygosity in transgenic plants. Distinguishing between plants that harbor one and two copies …

Agrobacterium-mediated transformation is widely used for gene delivery in plants. However,
commercial cultivars of crop plants are often recalcitrant to transformation because the …

Biotechnology provides plant breeders an additional tool to improve various traits desired by
growers and consumers of horticultural crops. It also provides genetic solutions to major …

In this paper, we report a novel pentatricopeptide repeat (PPR) protein gene in rice. PPR, a
characteristic repeat motif consisted of tandem 35 amino acids, has been found in various …

Wheat is one of the major staple food crops grown worldwide; however, productivity in
cereal crops has not kept pace with the world population growth. A significant increase in …

A number of factors that are known to influence genetic transformation were evaluated to
optimize Agrobacterium-mediated transformation of hypocotyl explants of cauliflower variety …

Most plastid transformation studies with tobacco, and all reports for other species (except
tomato [GD Nugent, M. ten Have, A. van der Gulik, PJ Dix, BA Uijtewaal, AP Mordhorst …

Glyphosate is a non-selective broad-spectrum herbicide that inhibits 5-enolpyruvylshikimate-
3-phosphate synthase (EPSPS). This is a key enzyme in the aromatic amino acid …

High necrosis and poor survival rate of target plant tissues are some of the major factors that
affect the efficiency of Agrobacterium-mediated T-DNA transfer into plant cells. These factors …