Mosquito species, members of the genera Aedes, Anopheles and Culex, are the major
vectors of human pathogens including protozoa (Plasmodium sp.), filariae and of a variety of …

Insect-borne diseases cause significant human morbidity and mortality. Current control and
preventive methods against vector-borne diseases rely mainly on insecticides. The …

The mosquito Aedes aegypti is a potent vector of the chikungunya, yellow fever, and dengue
viruses, responsible for hundreds of millions of infections and over 50,000 human deaths …

The development of CRISPR/Cas9 technologies has dramatically increased the accessibility
and efficiency of genome editing in many organisms. In general, in vivo germline expression …

Abstract piggyBac is a short inverted-repeat-type DNA transposable element originally
isolated from the genome of the moth Trichoplusia ni. It is currently the gene vector of choice …

Germ-line transformation of insects is now possible with four independent transposable
element vector systems. Among these, the TTAA-insertion site specific transposon …

Functional analysis of the Plasmodium falciparum genome is restricted because of the
limited ability to genetically manipulate this important human pathogen. We have developed …

Current techniques for the genetic engineering of insect genomes utilize transposable
genetic elements, which are inefficient, have limited carrying capacity and give rise to …

Background The Asian tiger mosquito, Aedes albopictus (Skuse), is a vector of several
arboviruses including dengue and chikungunya. This highly invasive species originating …

Stable and efficient germ‐line transformation was achieved in the South American malaria
vector, Anopheles albimanus, using a piggyBac vector marked with an enhanced green …