[HTML][HTML] Partial purification of crude lipase extract from Yarrowia lipolytica: precipitation, aqueous two-phase systems (ATPS), and immobilization methods

AIS Brígida, FS Buarque, VLR Nogueira… - Cleaner Chemical …, 2023 - Elsevier
Efforts have been concentrated on developing alternative methods of enzyme purification
that are less costly and highly efficient. In this work, we evaluated three different methods for …

Partitioning of pequi seed (Caryocar brasiliense Camb.) lipase in aqueous two-phase systems composed of PEG/2-propanol + ammonium sulfate + water

PA Nascimento, AN Alves, KA dos Santos… - Brazilian Journal of …, 2021 - Springer
Abstract Lipases (EC 3.1. 1.3) are biological catalysts widely used in industry due to their
versatility, selectivity and specificity. For this reason, the interest in their purification has …

Co-Expression of a Thermally Stable and Methanol-Resistant Lipase and Its Chaperone from Burkholderia cepacia G63 in Escherichia coli

J Zhang, M Tian, X Chen, P Lv, W Luo, Z Wang… - Applied Biochemistry …, 2021 - Springer
Biodiesel biosynthesis with enzymatic transesterification is considered green, sustainable,
and environmentally friendly method. Lipase from Burkholderia cepacia G63 has excellent …

Enantioselective synthesis of a chiral intermediate of himbacine analogs by Burkholderia cepacia lipase A

Y Chen, F Gao, G Zheng, S Gao - Biotechnology Letters, 2020 - Springer
The enantiomers of (4 R/S)-4-hydroxy-N, N-diphenyl-2-pentynamide are key chiral synthons
for the synthesis of thrombin receptor antagonists such as vorapaxar. In this paper, we report …

[PDF][PDF] Processos de separação e purificação de proteínas de origem vegetal: uma revisão

PA Nascimento, AN Alves, RCF Bonomo - downloads.editoracientifica.com.br
As proteínas extraídas de fontes vegetais têm se mostrado adequadas para aplicação em
processos industriais. Entretanto, é importante levar em consideração que seu uso por parte …

[PDF][PDF] The effects of diketopiperazines on the virulence of Burkholderia cepacia complex species

NM Jervis - 2021 - prism.ucalgary.ca
Recognized as a novel class of quorum sensing inhibitors (QSIs), 2, 5-diketopiperazines
(DKPs) are small, organic molecules that hold many important physiochemical properties …