There is a critical shortage in the number of deceased human organs that become available
for the purposes of clinical transplantation. This problem might be resolved by the …

Intracellular delivery is considered an indispensable process for various studies, ranging
from medical applications (cell‐based therapy) to fundamental (genome‐editing) and …

Background Clinical xenotransplantation is not possible because humans possess
antibodies that recognize antigens on the surface of pig cells. Galα‐1, 3‐G al (G al) and N …

The immunologic barriers to successful xenotransplantation are related to the presence of
natural anti‐pig antibodies in humans and non‐human primates that bind to antigens …

Cytoplasmic microinjection (CI) of the CRISPR/Cas9 system enabled the induction of site-
specific mutations in porcine zygotes and resulting pigs. However, mosaicism is a serious …

Cooper DKC, Ekser B, Burlak C, Ezzelarab M, Hara H, Paris L, Tector AJ, Phelps C,
Azimzadeh AM, Ayares D, Robson SC, Pierson RN III. Clinical lung xenotransplantation …

Recently, we established the GEEP (“gene editing by electroporation of Cas9 protein”)
method, in which the CRISPR/Cas9 system, consisting of a Cas9 protein and single guide …

锌指核酸酶(ZFN) 由锌指蛋白(ZFP) 结构域和Fok I 核酸内切酶的切割结构域人工融合而成,
是近年来发展起来的一种可用于基因组定点改造的分子工具. ZFN 可识别并结合特定的DNA …

Contents Somatic cell nuclear transfer (SCNT) was first developed in livestock for the
purpose of accelerating the widespread use of superior genotypes. Although many …

Background It is important to find out what would be the level of acceptance of
xenotransplantation in society, especially in populations where there are preclinical trials …