On the basis of phylogenetic analysis of nucleotide sequences, multiple genotypes and
subtypes of hepatitis C virus (HCV) have been identified. Characterization of these genetic …

The polymerase chain reaction (PCR) provides a simple, ingenious method to exponentially
amplify specific DNA sequences by in vitro DNA synthesis. Three essential steps to PCR …

Hepatitis C virus (HCV) shows substantial nucleotide sequence diversity distributed
throughout the viral genome, with many variants showing only 68 to 79% overall sequence …

Two viruses, GB virus A (GBV-A) and GB virus B (GBV-B), were recently identified in the GB
hepatitis agent. Human sera containing antibodies that recognize GBV-A and/or GBV-B …

We have succeeded in constructing a stable full-length cDNA clone of strain H77 (genotype
1a) of hepatitis C virus (HCV). We devised a cassette vector with fixed 5′ and 3′ termini …

Some individuals infected with hepatitis C virus (HCV) experience multiple episodes of
acute hepatitis. It is unclear whether these episodes are due to reinfection with HCV or to …

Because of the nucleotide sequence diversity of different isolates of hepatitis C virus, it has
become important to clarify whether distinct genotypes of hepatitis C virus vary with respect …

We have determined the nucleotide sequence of the 5'noncoding (NC) region of the
hepatitis C virus (HCV) genome in 44 isolates from around the world. We have identified …

Liver disease is an important cause of morbidity and mortality in patients with chronic renal
failure treated by dialysis and transplantation [1—101. Elevated serum alanine …

Diagnostic tests for hepatitis C can be divided into the following two general categories: 1)
serological assays that detect antibody to hepatitis C virus (anti‐HCV); and 2) molecular …