Metabolic engineering aims to develop efficient cell factories by rewiring cellular
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …

Highlights•Yeast is a major producer of biopharmaceutical proteins.•Rational metabolic
engineering is an efficient approach for strain improvement.•Systematically understanding …

One-step multiple gene disruption in the model organism Saccharomyces cerevisiae is a
highly useful tool for both basic and applied research, but it remains a challenge. Here, we …

Production of fuels and chemicals by industrial biotechnology requires efficient, safe and
flexible cell factory platforms that can be used for production of a wide range of compounds …

Many metabolic engineering and genetic engineering applications in yeast rely on the use
of plasmids. Despite their pervasive use and the diverse collections available, there is a …

Synthetic biology may be viewed as an effort to establish, formalize, and develop an
engineering discipline in the context of biological systems. The ability to tune the properties …

Improving the growth phenotypes of microbes in high product concentrations is an essential
design objective in the development of robust cell factories. However, the limited knowledge …

Hydroxypropionic acid (3-HP) is an attractive platform chemical, which can be used to
produce a variety of commodity chemicals, such as acrylic acid and acrylamide. For …

As a key intracellular metabolite, acetyl-coenzyme A (acetyl-CoA) plays a major role in
various metabolic pathways that link anabolism and catabolism. In the yeast Saccharomyces …

Background Due to an increasing demand of transportation fuels, a lower availability of
cheap crude oil and a lack of sustainability of fossil fuels, a gradual shift from petroleum …