Single-particle cryo-electron microscopy (cryo-EM SPA) has recently emerged as an
exceptionally well-suited technique for determining the structure of membrane proteins …

Reconstruction maps of cryo–electron microscopy (cryo-EM) exhibit distortion when the cryo-
EM dataset is incomplete, usually caused by unevenly distributed orientations. Prior efforts …

Cryo-electron microscopy (cryoEM) has become a well established technique to elucidate
the 3D structures of biological macromolecules. Projection images from thousands of …

We propose two new measures of resolution anisotropy for cryogenic electron microscopy
maps: Fourier shell occupancy (FSO), and the Bingham test (BT). FSO varies from 1 to 0 …

Xmipp is an open-source software package consisting of multiple programs for processing
data originating from electron microscopy and electron tomography, designed and managed …

Cryogenic electron tomography (cryoET) is a powerful tool in structural biology, enabling
detailed 3D imaging of biological specimens at a resolution of nanometers. Despite its …

Biomolecular complexes and their interactions govern cellular structure and function.
Understanding their architecture is a prerequisite for dissecting the cell's inner workings, but …

Photosystem I (PSI) is one of two photosystems involved in oxygenic photosynthesis. PSI of
cyanobacteria exists in monomeric, trimeric, and tetrameric forms, in contrast to the strictly …

Three-dimensional (3D) reconstruction in single-particle cryo-electron microscopy (cryo-EM)
is a significant technique for recovering the 3D structure of proteins or other biological …

Single-particle analysis (SPA) in cryo-electron microscopy has become a powerful tool for
determining and studying the macromolecular structure at an atomic level. However, since …