Cellular incorporation of unnatural amino acids and bioorthogonal labeling of proteins

K Lang, JW Chin - Chemical reviews, 2014 - ACS Publications
In the past 15 years, there have been rapid developments in the ability to cotranslationally
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …

Recent advances in biocatalysis with chemical modification and expanded amino acid alphabet

AD Pagar, MD Patil, DT Flood, TH Yoo… - Chemical …, 2021 - ACS Publications
The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

Residue-specific incorporation of non-canonical amino acids into proteins: recent developments and applications

JA Johnson, YY Lu, JA Van Deventer… - Current opinion in chemical …, 2010 - Elsevier
Residue-specific incorporation of non-canonical amino acids into proteins allows facile
alteration and enhancement of protein properties. In this review, we describe recent …

Cleavable biotin probes for labeling of biomolecules via azide− alkyne cycloaddition

J Szychowski, A Mahdavi, JJL Hodas… - Journal of the …, 2010 - ACS Publications
The azide− alkyne cycloaddition provides a powerful tool for bio-orthogonal labeling of
proteins, nucleic acids, glycans, and lipids. In some labeling experiments, eg, in proteomic …

Noncanonical amino acids in the interrogation of cellular protein synthesis

JT Ngo, DA Tirrell - Accounts of chemical research, 2011 - ACS Publications
Proteins in living cells can be made receptive to bioorthogonal chemistries through
metabolic labeling with appropriately designed noncanonical amino acids (ncAAs). In the …

Peptide-directed self-assembly of hydrogels

J Kopeček, J Yang - Acta biomaterialia, 2009 - Elsevier
This review focuses on the self-assembly of macromolecules mediated by the biorecognition
of peptide/protein domains. Structures forming α-helices and β-sheets have been used to …

Cell-selective metabolic labeling of proteins

JT Ngo, JA Champion, A Mahdavi… - nature CHeMICaL …, 2009 - nature.com
Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be
targeted exclusively to specified cells through expression of a mutant methionyl-tRNA …

Genetic Code Expansion Approaches to Decipher the Ubiquitin Code

V Wanka, M Fottner, M Cigler, K Lang - Chemical Reviews, 2024 - ACS Publications
The covalent attachment of Ub (ubiquitin) to target proteins (ubiquitylation) represents one of
the most versatile PTMs (post-translational modifications) in eukaryotic cells. Substrate …

Engineered Proteins and Materials Utilizing Residue-Specific Noncanonical Amino Acid Incorporation

T Majekodunmi, D Britton, JK Montclare - Chemical reviews, 2024 - ACS Publications
The incorporation of noncanonical amino acids into proteins and protein-based materials
has significantly expanded the repertoire of available protein structures and chemistries …

Biocatalysts by evolution

C Jäckel, D Hilvert - Current opinion in biotechnology, 2010 - Elsevier
Proteins evolve by iterative cycles of mutation, selection and amplification. Analogous
evolutionary strategies are being profitably exploited in the laboratory to generate and …