The emerging and uncultivated potential of CRISPR technology in plant science

Y Zhang, AA Malzahn, S Sretenovic, Y Qi - Nature Plants, 2019 - nature.com
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

[HTML][HTML] CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation

T Cardi, J Murovec, A Bakhsh, J Boniecka… - Trends in Plant …, 2023 - cell.com
The discovery of the CRISPR/Cas genome-editing system has revolutionized our
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …

Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture

T Li, J Hu, Y Sun, B Li, D Zhang, W Li, J Liu, D Li… - Molecular Plant, 2021 - cell.com
Genome editing provides novel strategies for improving plant traits but mostly relies on
conventional plant genetic transformation and regeneration procedures, which can be …

Engineered biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes

Q Liu, C Zhao, K Sun, Y Deng, Z Li - Molecular Plant, 2023 - cell.com
CRISPR/Cas genome-editing tools provide unprecedented opportunities for basic plant
biology research and crop breeding. However, the lack of robust delivery methods has …

Plant genome editing with TALEN and CRISPR

A Malzahn, L Lowder, Y Qi - Cell & bioscience, 2017 - Springer
Genome editing promises giant leaps forward in advancing biotechnology, agriculture, and
basic research. The process relies on the use of sequence specific nucleases (SSNs) to …

Potato virus X vector-mediated DNA-free genome editing in plants

H Ariga, S Toki, K Ishibashi - Plant and Cell Physiology, 2020 - academic.oup.com
Genome editing technology is important for plant science and crop breeding. Genome-
edited plants prepared using general CRISPR-Cas9 methods usually contain foreign DNA …

Genome editing—principles and applications for functional genomics research and crop improvement

H Zhang, J Zhang, Z Lang, JR Botella… - Critical Reviews in Plant …, 2017 - Taylor & Francis
Genome editing technologies are powerful tools for studying gene function and for crop
improvement. The technologies rely on engineered endonucleases to generate double …

Exploration of plant-microbe interactions for sustainable agriculture in CRISPR era

RM Shelake, D Pramanik, JY Kim - Microorganisms, 2019 - mdpi.com
Plants and microbes are co-evolved and interact with each other in nature. Plant-associated
microbes, often referred to as plant microbiota, are an integral part of plant life. Depending …

Virus-induced plant genome editing

Y Oh, H Kim, SG Kim - Current opinion in plant biology, 2021 - Elsevier
Plant viruses have been engineered to express heterologous proteins and RNAs in plants
for several decades. This viral system can now be applied to editing plant genomes. Virus …

Plant virus-derived vectors for plant genome engineering

MA Mahmood, RZ Naqvi, SU Rahman, I Amin… - Viruses, 2023 - mdpi.com
Advances in genome engineering (GE) tools based on sequence-specific programmable
nucleases have revolutionized precise genome editing in plants. However, only the …