In-cell structural biology by NMR: the benefits of the atomic scale

FX Theillet - Chemical reviews, 2022 - ACS Publications
In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Thermodynamic forces from protein and water govern condensate formation of an intrinsically disordered protein domain

S Mukherjee, LV Schäfer - Nature Communications, 2023 - nature.com
Liquid-liquid phase separation (LLPS) can drive a multitude of cellular processes by
compartmentalizing biological cells via the formation of dense liquid biomolecular …

Biological colloids: Unique properties of membraneless organelles in the cell

A Bratek-Skicki, M Van Nerom, D Maes… - Advances in Colloid and …, 2022 - Elsevier
Biomolecular condensates are membraneless, intracellular organelles that form via liquid-
liquid phase separation (LLPS) and have the ability to concentrate a wide range of …

Biomolecular condensates modulate membrane lipid packing and hydration

A Mangiarotti, M Siri, NW Tam, Z Zhao… - Nature …, 2023 - nature.com
Membrane wetting by biomolecular condensates recently emerged as a key phenomenon in
cell biology, playing an important role in a diverse range of processes across different …

Single-droplet surface-enhanced Raman scattering decodes the molecular determinants of liquid-liquid phase separation

A Avni, A Joshi, A Walimbe, SG Pattanashetty… - Nature …, 2022 - nature.com
Biomolecular condensates formed via liquid-liquid phase separation (LLPS) are involved in
a myriad of critical cellular functions and debilitating neurodegenerative diseases …

Capillary flow experiments for thermodynamic and kinetic characterization of protein liquid-liquid phase separation

EGP Stender, S Ray, RK Norrild, JA Larsen… - Nature …, 2021 - nature.com
Liquid-liquid phase separation or LLPS of proteins is a field of mounting importance and the
value of quantitative kinetic and thermodynamic characterization of LLPS is increasingly …

Modulation of Biomolecular Liquid–Liquid Phase Separation by Preferential Hydration and Interaction of Small Osmolytes with Proteins

K Kitamura, A Oshima, F Sasaki… - The Journal of …, 2024 - ACS Publications
We examined the effects of trimethylamine N-oxide (TMAO) and urea (known osmolytes) on
the liquid–liquid phase separation (LLPS) of fused in sarcoma (FUS) and three FUS-LLPS …

Regulating of Liquid–Liquid Phase Separation and Molecular Self-Assembly through Selective Solvation

Y Huang, N Wang, J Wang, X Ji, J Yang… - Industrial & …, 2023 - ACS Publications
Effectively regulating the liquid–liquid phase separation (LLPS) phenomenon in the process
of molecular self-assembly is critical for controlling the qualities of the final products …

Nucleobase clustering contributes to the formation and hollowing of repeat-expansion RNA condensate

Y Ma, H Li, Z Gong, S Yang, P Wang… - Journal of the American …, 2022 - ACS Publications
RNA molecules with repeat expansion sequences can phase separate into gel-like
condensate, which could lead to neurodegenerative diseases. Here, we report that, in the …

Concentration quantification of the low-complexity domain of fused in sarcoma inside a single droplet and effects of solution parameters

K Yokosawa, S Kajimoto, D Shibata… - The Journal of …, 2022 - ACS Publications
Liquid–liquid phase separation (LLPS) is an important phenomenon in biology, and it is
desirable to develop quantitative methods to analyze protein droplets generated by LLPS …