The major impediment to the development of murine monoclonal antibodies (mAbs) for
therapy in humans has been the difficulty in reducing their potential immunogenicity …

The ability to produce a diverse repertoire of fully human monoclonal antibodies may have
significant applications to human therapy. One of the most promising approaches to the …

INTRODUCTION of DNA fragments, hundreds of kilobases in size, into mouse embryonic
stem (ES) cells would greatly advance the ability to manipulate the mouse genome. Mice …

We have designed a P1 vector (pCYPAC–1) for the introduction of recombinant DNA into E.
coli using electroporation procedures. The new cloning system, P1–derived arteficial …

The yeast two-hybrid system is a molecular genetic test for protein interaction. Here we
describe a step by step procedure to screen for proteins that interact with a protein of interest …

Bacterial artificial chromosome (BAC) libraries have become invaluable tools in plant
genetic research. However, it is difficult for new practitioners to create plant BAC libraries de …

We have developed a strategy for the rapid enrichment and identification of cDNAs encoded
by large genomic regions. The basis of this" direct selection" scheme is the hybridization of …

The escape and migration of genetic information between mitochondria, chloroplasts, and
nuclei have been an integral part of evolution and has a continuing impact on the biology of …

A general strategy for cloning and mapping large regions of human DNA with yeast artificial
chromosomes (YAC's) is described. It relies on the use of the polymerase chain reaction to …

Methods for the construction of yeast artificial-chromosome (YAC) clones have been
designed to isolate single, large (100–1000 kb) segments of chromosomal DNA. It is …