Plant-based production of an orally active cyclotide for the treatment of multiple sclerosis

MA Jackson, J Xie, LTT Nguyen, X Wang, K Yap… - Transgenic …, 2023 - Springer
Multiple sclerosis (MS) is a debilitating disease that requires prolonged treatment with often
severe side effects. One experimental MS therapeutic currently under development is a …

Consensus design and engineering of an efficient and high-yield peptide asparaginyl ligase for protein cyclization and ligation

X Hemu, X Zhang, HY Chang, JE Poh… - Journal of Biological …, 2023 - ASBMB
Plant legumains are Asn/Asp-specific endopeptidases that have diverse functions in plants.
Peptide asparaginyl ligases (PALs) are a special legumain subtype that primarily catalyze …

Design of a recombinant asparaginyl ligase for site-specific modification using efficient recognition and nucleophile motifs

J Tang, M Hao, J Liu, Y Chen, G Wufuer, J Zhu… - Communications …, 2024 - nature.com
Asparaginyl ligases have been extensively utilized as valuable tools for site-specific
bioconjugation or surface-modification. However, the application is hindered by the …

Repurposing Amide Bond-Forming Enzymes for Non-native Protein Modification

T Zhu, B Wu - ACS Catalysis, 2024 - ACS Publications
Site-specific protein modification techniques have led to breakthroughs across diverse
fields, such as cell imaging, proteomics, biomaterials, and pharmaceutical protein and …

Consensus design and engineering of an efficient and high-yield Peptide Asparaginyl Ligase

X Hemu, X Zhang, HY Chang, PJ En, JP Tam - bioRxiv, 2022 - biorxiv.org
Abstract Plant legumains are Asn/Asp-specific endopeptidases (AEPs) that have diverse
functions in plants. Peptide asparaginyl ligases (PALs) are a special legumain subtype that …