The active transport of toxic compounds by membrane-bound efflux proteins is becoming an
increasingly frequent mechanism by which cells exhibit resistance to therapeutic drugs. This …

Cells regulate gene expression using networks of transcription interactions; it is of interest to
discover the principles that govern the dynamical behavior of such networks. An important …

Gene regulation by tetracyclines has become a widely-used tool to study gene functions in
pro-and eukaryotes. This regulatory system originates from Gram-negative bacteria, in which …

We report for the first time the in vitro characterization of a reverse tetracycline repressor
(revTetR). The dimeric wild‐type repressor (TetR) binds to tet operator tetO in the absence of …

We explore by extensive mutagenesis regions in the sequence allowing reversal of the
allosteric response of Tet repressor. The wild type requires anhydrotetracycline for induction …

The dramatically increasing prevalence of multi‐drug‐resistant human pathogenic bacteria
and related mortality requires two key actions:(i) decisive initiatives for the detection of novel …

Tet Repressor (TetR) recognizes the inducer tetracycline (tc) with high affinity. The tc analog
4-de (dimethylamino)-6-deoxy-6-demethyl-tetracycline (cmt3) is not an inducer for TetR …

New experimental findings continue to challenge our understanding of protein allostery.
Recent deep mutational scanning study showed that allosteric hotspots in the tetracycline …

Recently, allosteric transcription factors (TFs) were identified as a novel class of
biorecognition elements for in vitro sensing, whereby an indicator of the differential binding …

A recent description of an antibody‐free assay is significantly extended for small molecule
analytes using allosteric transcription factors (aTFs) and Förster resonance energy transfer …