The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert
DNA cassettes into eukaryotic chromosomes is widespread (1–9). Indeed, a PubMed search …

How life began still eludes science life, the initial progenote in the context presented herein,
being a chemical aggregate of primordial inorganic and organic molecules capable of self …

Members of the tyrosine recombinase (YR) family of site-specific recombinases catalyze
DNA rearrangements using phosphoryl transfer chemistry that is identical to that used by the …

Tyrosine family site‐specific recombinases (YRs), named after the active site tyrosine
nucleophile they utilize for DNA strand breakage, are widely distributed among prokaryotes …

Summary Selfish genetic elements (1–4), widespread in nature, are characterized by their
ability to replicate efficiently and maintain themselves stably in host cell populations. A …

The Cre-recombination system has become an important tool for genetic manipulation of
higher organisms and a model for site-specific DNA-recombination mechanisms employed …

The E. coli chromosome is compacted by segregation into 400–500 supercoiled domains by
both active and passive mechanisms, for example, transcription and DNA-protein …

Restriction endonucleases are expressed in all bacteria investigated so far and play an
essential role for the bacterial defense against viral infections. Besides their important …

The active site of the tyrosine family site-specific recombinase Flp contains a conserved
catalytic pentad that includes two arginine residues, Arg-191 and Arg-308. Both arginines …

Two conserved catalytic arginines, Arg-173 and Arg-292, of the tyrosine site-specific
recombinase Cre are essential for the transesterification steps of strand cleavage and …