ABCs of DNA aptamer and related assay development

TK Sharma, JG Bruno, A Dhiman - Biotechnology advances, 2017 - Elsevier
This review is intended to guide the novice in aptamer research and development to
understand virtually all of the aptamer development options and currently available assay …

[HTML][HTML] Therapeutic applications of nucleic acid aptamers in microbial infections

S Afrasiabi, M Pourhajibagher, R Raoofian… - Journal of Biomedical …, 2020 - Springer
Today, the treatment of bacterial infections is a major challenge, due to growing rate of
multidrug-resistant bacteria, complication of treatment and increased healthcare costs …

Nucleic acid biosensor synthesis of an all-in-one universal blocking linker recombinase polymerase amplification with a peptide nucleic acid-based lateral flow device …

Y Xu, Y Wei, N Cheng, K Huang, W Wang… - Analytical …, 2018 - ACS Publications
In this study, a whole-course nucleic-acid-constructed biosensor that combines the all-in-
one concepts of the universal blocking linker recombinase polymerase amplification …

[HTML][HTML] Generation and Selection of Specific Aptamers Targeting Brucella Species through an Enhanced Cell-SELEX Methodology

DM El-Husseini, AE Sayour, F Melzer… - International Journal of …, 2022 - mdpi.com
Brucellae are Gram-negative, aerobic, non-motile coccobacilli causing brucellosis in man
and animals. The disease is one of the most significant yet neglected global zoonoses …

[HTML][HTML] Development of cell-specific aptamers: recent advances and insight into the selection procedures

K Rahimizadeh, H AlShamaileh, M Fratini… - Molecules, 2017 - mdpi.com
Systematic evolution of ligands by exponential enrichment (SELEX) is an established
procedure for developing short single-stranded nucleic acid ligands called aptamers against …

Essential strategies to optimize asymmetric PCR conditions as a reliable method to generate large amount of ssDNA aptamers

M Heiat, R Ranjbar, AM Latifi… - Biotechnology and …, 2017 - Wiley Online Library
Asymmetric PCR, a simple method to generate single‐stranded DNA (ssDNA) aptamers in
systematic evaluation of ligand by exponential enrichments rounds, is coupled with …

A simple modification increases specificity and efficiency of asymmetric PCR

Z Tolnai, Á Harkai, Z Szeitner, ÉN Scholz, K Percze… - Analytica Chimica …, 2019 - Elsevier
Although various methods have been developed to suffice the oligonucleotide demand of
molecular biology laboratories, in vitro production of high-purity ssDNAs remains to be a …

Development of an optimization pipeline of asymmetric PCR towards the generation of DNA aptamers: a guide for beginners

TS Yeoh, A Anna, TH Tang, M Citartan - World Journal of Microbiology …, 2022 - Springer
Asymmetric PCR is one of the most utilized strategies in ssDNA generation towards DNA
aptamer generation due to its low cost, robustness and the low amount of starting template …

[HTML][HTML] G-quadruplex-forming DNA aptamers inhibit the DNA-binding function of HupB and Mycobacterium tuberculosis entry into host cells

P Kalra, SK Mishra, S Kaur, A Kumar, HK Prasad… - … Therapy-Nucleic Acids, 2018 - cell.com
The entry and survival of Mycobacterium tuberculosis (Mtb) within host cells is orchestrated
partly by an essential histone-like protein HupB (Rv2986c). Despite being an essential drug …

[HTML][HTML] Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage

E Yoo, D Choe, J Shin, S Cho, BK Cho - Computational and Structural …, 2021 - Elsevier
The market for using and storing digital data is growing, with DNA synthesis emerging as an
efficient way to store massive amounts of data. Storing information in DNA mainly consists of …