Legionella and Legionnaires' Disease: 25 Years of Investigation

BS Fields, RF Benson, RE Besser - Clinical microbiology reviews, 2002 - Am Soc Microbiol
There is still a low level of clinical awareness regarding Legionnaires' disease 25 years after
it was first detected. The causative agents, legionellae, are freshwater bacteria with a …

Legionella spp. and Legionnaires' disease

BMW Diederen - Journal of infection, 2008 - Elsevier
Infection with Legionella spp. is an important cause of community-and hospital-acquired
pneumonia, occurring both sporadically and in outbreaks. Infection with Legionella spp …

Diagnosis of Legionella Infection

LB Reller, MP Weinstein… - Clinical infectious …, 2003 - academic.oup.com
Legionellae, which are important causes of pneumonia in humans, continue to be incorrectly
labeled as exotic pathogens. The ability to diagnose Legionella infection is limited by the …

Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR

N Wellinghausen, C Frost, R Marre - Applied and environmental …, 2001 - Am Soc Microbiol
Contamination of hospital water systems with legionellae is a well-known cause of
nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for …

Detection of Legionella Species in Respiratory Specimens Using PCR with Sequencing Confirmation

JL Cloud, KC Carroll, P Pixton, M Erali… - Journal of clinical …, 2000 - Am Soc Microbiol
Legionella spp. are a common cause of community-acquired respiratory tract infections and
an occasional cause of nosocomial pneumonia. A PCR method for the detection of …

Development of a new seminested PCR method for detection of Legionella species and its application to surveillance of legionellae in hospital cooling tower water

H Miyamoto, H Yamamoto, K Arima, J Fujii… - Applied and …, 1997 - Am Soc Microbiol
The presence of PCR inhibitors in water samples is well known and contributes to the fact
that a practical PCR assay has not been developed for legionella surveillance. In this study …

Determining the microbial and chemical contamination in Ecuador's main rivers

D Vinueza, V Ochoa-Herrera, L Maurice, E Tamayo… - Scientific reports, 2021 - nature.com
One major health issue is the microbial and chemical contamination of natural freshwater,
particularly in Latin American countries, such as Ecuador, where it is still lacking wastewater …

Direct Detection of Legionella Species from Bronchoalveolar Lavage and Open Lung Biopsy Specimens: Comparison of LightCycler PCR, In Situ Hybridization …

RT Hayden, JR Uhl, X Qian, MK Hopkins… - Journal of clinical …, 2001 - Am Soc Microbiol
We developed a rapid thermocycling, real-time detection (also known as real-time PCR)
method for the detection of Legionella species directly from clinical specimens. This method …

Quantitative Real-Time Legionella PCR for Environmental Water Samples: Data Interpretation

P Joly, PA Falconnet, J André, N Weill… - Applied and …, 2006 - Am Soc Microbiol
ABSTRACT Quantitative Legionella PCRs targeting the 16S rRNA gene (specific for the
genus Legionella) and the mip gene (specific for the species Legionella pneumophila) were …

Diagnosis of Legionella infection in Legionnaires' disease

JW Den Boer, EPF Yzerman - European Journal of Clinical Microbiology …, 2004 - Springer
Since 1977, the diagnostic tools for Legionnaires' disease have been culture and
serological investigation. Both methods require considerable time to produce results and …