To survive low temperature is required for a long-term storage (cryopreservation), cells
should be vitrified to a state in which intracellular water is solidified without ice crystal …

Artificial insemination (AI) with cryopreserved semen is an important tool to preserve
endangered species, including European donkey breeds. Sperm vitrification is an …

Sperm cryopreservation by ultra-rapid cooling based on dropping small volumes of sperm
suspension directly into liquid nitrogen, has been successful in some wild ruminant species …

In this study, we successfully described for the first time a vitrification of epididymal Iberian
ibex spermatozoa. Spermatozoa from epididymis were obtained from 15 Iberian ibex. The …

The present study compares a protocol that mimics freezing of ram semen in static nitrogen
vapor with two protocols with an initial low cooling rate in the first step, followed by higher …

A method for cryopreserving wild ibex sperm at high cooling rates was developed. To design
a freezing solution based on Tris, citric acid, and glucose (TCG), two preliminary …

The cryopreservation of ejaculate can reduce the viability, motility, and morphological
characteristics of the spermatozoa of infertile men. Oligoasthenoteratozoospermia (OAT) is …

This work examines the effect of equilibration time with extender on ultra-rapidly frozen-
thawed wild ruminant epididymal (origin: Iberian ibex) and ejaculated (origin: mouflon) …

Nowadays, human oocytes/embryos are cryopreserved via slow freezing or vitrification. The
aim of this study was to evaluate a rapid warming protocol for slow-frozen human oocytes …

Sperm vitrification is a rapid freezing method in which carbohydrates are used as
cryoprotectants. The aim of this study was to determine the optimal volume, concentration …