Starting in 1991, the advance of Tyr-recombinases Flp and Cre enabled superior strategies
for the predictable insertion of transgenes into compatible target sites of mammalian cells …

Traditional DNA transduction routes used for the modification of cellular genomes are
subject to unpredictable alterations, as the cell-intrinsic repair machinery may affect both the …

Interleukin-7 receptor α (encoded by IL7R) is essential for lymphoid development. Whether
acute lymphoblastic leukemia (ALL)-related IL7R gain-of-function mutations can trigger …

The acquired gain-of-function V617F mutation in the Janus Kinase 2 (JAK2V617F) is the
main mutation involved in BCR/ABL-negative myeloproliferative neoplasms (MPNs), but its …

The human SLC6 family is composed of approximately 20 structurally related symporters (co-
transporters) that use the transmembrane electrochemical gradient to actively import their …

After the successful completion of the human genome project (HGP), biological research in
the postgenome era urgently needs an efficient approach for functional analysis of genes …

Arterial cardiovascular events are the leading cause of death in patients with JAK2V617F
myeloproliferative neoplasms (MPNs). However, their mechanisms are poorly understood …

Auditory sensory hair cells depend on stereocilia with precisely regulated lengths to detect
sound. Since stereocilia are primarily composed of crosslinked, parallel actin filaments …

Abstract JAK2V617F mutation is associated with an increased risk for athero-thrombotic
cardiovascular disease, but its role in aortic disease development and complications …

Site-specific recombination systems are widespread and popular tools for all scientists
interested in manipulating the mouse genome. In this chapter, we focus on the use of site …