The discovery, only a decade ago, of the genome editing power of clustered regularly interspaced short palindromic repeats (CRISPR)-associated nucleases is already …
JR Davis, X Wang, IP Witte, TP Huang… - Nature Biomedical …, 2022 - nature.com
The viral delivery of base editors has been complicated by their size and by the limited packaging capacity of adeno-associated viruses (AAVs). Typically, dual-AAV approaches …
Y Wang, T Qi, J Liu, Y Yang, Z Wang, Y Wang… - Science …, 2023 - science.org
The CRISPR-Cas system can treat autosomal dominant diseases by nonhomologous end joining (NHEJ) gene disruption of mutant alleles. However, many single-nucleotide …
Adeno-associated virus (AAV) vectors are important delivery platforms for therapeutic genome editing but are severely constrained by cargo limits. Simultaneous delivery of …
CRISPR–Cas9 genome engineering is a powerful technology for correcting genetic diseases. However, the targeting range of Cas9 proteins is limited by their requirement for a …
H Zeng, Q Yuan, F Peng, D Ma, A Lingineni… - Nature …, 2023 - nature.com
DNA base editors use deaminases fused to a programmable DNA-binding protein for targeted nucleotide conversion. However, the most widely used TadA deaminases lack post …
M Liu, W Zhang, C Xin, J Yin, Y Shang, C Ai… - Nucleic acids …, 2021 - academic.oup.com
CRISPR–Cas9 generates double-stranded DNA breaks (DSBs) to activate cellular DNA repair pathways for genome editing. The repair of DSBs leads to small insertions or …
DY Kim, Y Chung, Y Lee, D Jeong, KH Park… - Nature chemical …, 2022 - nature.com
Cas12f is a hypercompact type V, Cas12 family member. Previously, we reported a set of engineered guide RNAs supporting high indel efficiency for Cas12f1 in human cells. Here …
SH Chu, M Packer, H Rees, D Lam, Y Yu… - The CRISPR …, 2021 - liebertpub.com
Base editors are fusions of a deaminase and CRISPR-Cas ribonucleoprotein that allow programmable installment of transition mutations without double-strand DNA break …