Phospholipids play multiple roles in cells by establishing the permeability barrier for cells
and cell organelles, by providing the matrix for the assembly and function of a wide variety of …

Chromosome replication in Escherichia coli is normally initiated at oriC, the origin of
chromosome replication. E. coli cells possess at least three additional initiation systems for …

A multiprotein complex that specifically recognizes cellular origins of DNA replication has
been identified and purified from the yeast Saccharomyces cerevisiae. We observe a strong …

Homologous recombination can be used to generate recombinants on episomes or directly
on the Escherichia coli chromosome with PCR products or synthetic single-stranded DNA …

The initiation of replication is the central event in the bacterial cell cycle. Cells control the
rate of DNA synthesis by modulating the frequency with which new chains are initiated, like …

▪ Abstract In the past few years, in vivo technologies have emerged that, due to their
efficiency and simplicity, may one day replace standard genetic engineering techniques …

Equilibrium and kinetic rate constants were determined for the binding of the initiator protein
DnaA of Escherichia coli to its binding site, the non-palindromic 9-bp DnaA box, using gel …

This review summarizes the mechanisms of the initiator protein DnaA in replication initiation
and its regulation in Escherichia coli. The chromosomal origin (oriC) DNA is unwound by the …

ATP binding to dnaA protein is essential for its action in initiating the replication of plasmids
that bear the unique origin of the Escherichia coli chromosome (oriC). ADP bound to that site …

The growth of E. coli cells into a critical mass results in the formation of G-ring of a GTP-
binding protein (FtsZ) around the cell center. Chromosomal DNA replication is initiated at a …