Electron microscopy played a key role in establishing cell biology as a discipline, by
producing fundamental insights into cellular organization and ultrastructure. Many seminal …

Highlights•Whole-cell and on-lamellae cryo-ET for structural studies of viral
infection.•Inactivation protocols for improved structural preservation and biosafety.•VLP and …

During its cytoplasmic replication, vaccinia virus assembles non-infectious spherical
immature virions (IV) coated by a viral D13 lattice. Subsequently, IV mature into infectious …

The CytoFLEX is a novel semiconductor-based flow cytometer that utilizes avalanche
photodiodes, wavelength-division multiplexing, enhanced optics, and diode lasers to …

Several major human pathogens, including the filoviruses, paramyxoviruses, and
rhabdoviruses, package their single-stranded RNA genomes within helical nucleocapsids …

Poxviruses are considered to be unique among all DNA viruses, because their infection
cycle is carried out exclusively in the host cytoplasm. Such an infection strategy is of interest …

Cryo-electron tomography (cryo-ET) allows for the visualization of biological material in a
close-to-native state, in three dimensions and with nanometer scale resolution. However …

Host cell entry of vaccinia virus, the prototypic poxvirus, involves a membrane fusion event
delivering the viral core and two proteinaceous lateral bodies (LBs) into the cytosol …

The standard pathway for virus infection of eukaryotic cells requires disassembly of the viral
shell to facilitate release of the viral genome into the host cell. Here we use mechanical …

We have performed full-field cryo X-ray microscopy in the water window photon energy
range on vaccinia virus (VACV) infected cells to produce tomographic reconstructions. PtK2 …