Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …

With both catalytic and genetic functions, ribonucleic acid (RNA) is perhaps the most
pluripotent chemical species in molecular biology, and its functions are intimately linked to …

INTRODUCTION In DNA double helices, hydrogen bonds connect the base pairs across the
two strands, and stacking bonds act along the helical axis between neighboring base pairs …

originally designed to reproduce the properties of DNA molecules with average sequences.
The new parametrization introduces sequence-dependent stacking and base-pairing …

uMelt SM is a flexible web-based tool for predicting DNA melting curves and denaturation
profiles of PCR products. The user defines an amplicon sequence and chooses a set of …

Folding may be described conceptually in terms of trajectories over a landscape of free
energies corresponding to different molecular configurations. In practice, energy landscapes …

The relationship between protein three-dimensional structure and function is essential for
mechanism determination. Unfortunately, most techniques do not provide a direct …

It has been known for decades that DNA is extremely flexible and polymorphic, but our
knowledge of its accessible conformational space remains limited. Structural data, primarily …

To simulate long time and length scale processes involving DNA it is necessary to use a
coarse-grained description. Here we provide an overview of different approaches to such …

SMC complexes play key roles in genome maintenance, where they ensure efficient
genome replication and segregation. The SMC complex Smc5/6 is a crucial player in DNA …