The ability of many microorganisms to grow at high temperatures has held a particular
fascination for microbiologists and biochemists since a long time. As any of their cellular …

Immunoglobulin variable domains are generally thought of as well conserved platforms
providing the base for antigen binding loops of highly varying sequence and structure …

The very term “protein engineering” remains something of an oxymoron, at least as far as
engineering implies the rational application of well understood principles towards achieving …

Limited thermostability of antibiotic resistance markers has restricted genetic research in the
field of extremely thermophilic Archaea and bacteria. In this study, we used directed …

60oC for 1 hour was determined in a buffer containing 50 mM Tris, pH 7.8, 1 mM 5, 5'-
dithiobis-(2-nitrobenzoic acid)(DTNB, Pierce), 0.1 mM chloramphenicol, and 0.4 mM acetyl …

Chitinases with high thermostability are important for many industrial and biotechnological
applications. This study was conducted to enhance the stability of Serratia marcescens B4A …

Enzymatic decarboxylation of biobased hydroxycinnamic acids gives access to phenolic
styrenes for adhesive production. Phenolic acid decarboxylases are proficient enzymes that …

[57] ABSTRACT A process for improving the stability of an antibody or fragment thereof is
disclosed. A gene encoding for an amino acid sequence of a variable domain of an antibody …

We established a Thermus thermophilus strain in which the pyrE gene (coding for orotate
phosphoribosyltransferase of the pyrimidine biosynthetic pathway) was totally deleted. We …

Abstract Hyperthermophilic Archaea and Bacteria are an extraordinarily important class of
organisms for which genetic tools remain to be developed. Unique technological obstacles …