Mass spectrometry (MS) has become one of the key technologies of structural biology. In this
review, the contributions of chemical cross-linking combined with mass spectrometry (XL …

Biological phase separation is known to be important for cellular organization, which has
recently been extended to a new class of biomolecules that form liquid-like droplets …

Recognition of the natural abundance and functional importance of intrinsically disordered
proteins (IDPs), and protein hybrids that contain both intrinsically disordered protein regions …

Intrinsically disordered protein regions (IDRs)-regions that do not fold into a fixed three-
dimensional structure but instead exist in a heterogeneous ensemble of conformations-have …

Many proteins and protein segments cannot attain a single stable three-dimensional
structure under physiological conditions; instead, they adopt multiple interconverting …

Highlights•Not all IDRs undergo liquid–liquid phase separation.•IDRs are not necessary for
protein phase separation.•A stickers-and-spacers framework helps conceptualizing IDR …

Intrinsically disordered proteins (IDPs) are important components of the cellular signalling
machinery, allowing the same polypeptide to undertake different interactions with different …

Protein intrinsic disorder is found in all kingdoms of life and is known to underpin numerous
physiological and pathological processes. Computational methods play an important role in …

Intrinsically disordered proteins (IDPs) are unable to adopt a unique 3D structure under
physiological conditions and thus exist as highly dynamic conformational ensembles. IDPs …

Despite the wealth of knowledge gained about intrinsically disordered proteins (IDPs) since
their discovery, there are several aspects that remain unexplored and, hence, poorly …