It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …

Interleukin-2 (IL-2), the first cytokine that was molecularly cloned, was shown to be a T cell
growth factor essential for the proliferation of T cells and the generation of effector and …

The poor correlation of mutational landscapes with phenotypes limits our understanding of
the pathogenesis and metastasis of pancreatic ductal adenocarcinoma (PDAC). Here we …

Pancreatic ductal adenocarcinoma (PDA) is notoriously aggressive and hard to treat. The
tumour microenvironment (TME) in PDA is highly dynamic and has been found to promote …

Pancreatic cancer is a major cause of cancer-associated mortality, with a dismal overall
prognosis that has remained virtually unchanged for many decades. Currently, prevention or …

T cell non-Hodgkin lymphomas are a heterogeneous group of highly aggressive
malignancies with poor clinical outcomes. T cell lymphomas originate from peripheral T cells …

Transposon insertion sequencing is a high-throughput technique for assaying large libraries
of otherwise isogenic transposon mutants providing insight into gene essentiality, gene …

Mouse transgenesis has provided fundamental insights into pancreatic cancer, but is limited
by the long duration of allele/model generation. Here we show transfection-based …

DNA transposons offer an efficient nonviral method of permanently modifying the genomes
of mammalian cells. The piggyBac transposon system has proven effective in genomic …

With the genetic portraits of all major human malignancies now available, we next face the
challenge of characterizing the function of mutated genes, their downstream targets …