Gene therapy offers great opportunities for the treatment of severe diseases including
cancer. In recent years the design of synthetic carriers for nucleic acid delivery has become …

Recombinant adeno-associated viruses (rAAVs) are among the most important vectors for in
vivo gene therapies. With the rapid development of gene therapy, current rAAV …

Manufacturing of recombinant adeno-associated virus (rAAV) viral vectors remains
challenging, with low yields and low full: empty capsid ratios in the harvest. To elucidate the …

Natural and synthetic biological networks must function reliably in the face of fluctuating
stoichiometry of their molecular components. These fluctuations are caused in part by …

In artificial gene delivery, messenger RNA (mRNA) is an attractive alternative to plasmid
DNA (pDNA) since it does not require transfer into the cell nucleus. Here we show that …

Lipid/polymer hybrid nanoparticles loaded with red fluorescent protein (RFP) encoded
plasmid DNA (pDNA) was formulated using poly-lactic-co-glycolic acid (PLGA), cationic lipid …

Cell-free in vitro expression is increasingly important for high-throughput expression
screening, high yield protein production and synthetic biology applications. Yet its potential …

Despite many efforts in the rational design of nanoparticles (NPs) to overcome the biological
barriers to small interfering RNA (siRNA) delivery for improving gene silencing efficiency …

Steric stabilization of cationic liposome–DNA (CL–DNA) complexes is required for in vivo
applications such as gene therapy. PEGylation (PEG: poly (ethylene glycol)) of CL–DNA …

Molecular-level information processing, is essential for 'smart'in vivo nanosystems. Natural
molecular computing, such as the regulation of messenger RNA (mRNA) synthesis by …