Based on the antimalarial properties observed for 2-acetylpyridine 4-phenyl-3-
thiosemicarbazone (1), an extensive series of related thiosemicarbazones was prepared …

Materials and Methods Folate, FAH2, and pronase were obtained from Sigma Chemical Co.
Pteroic acid, homofolate, methotrexate, dichloromethotrexate, and 5-fluorodeoxyuridine …

X-ray data have been extended to 1.7 A for a binary complex of Escherichia coli
dihydrofolate reductase with methotrexate and a ternary complex of Lactobacillus casei …

The atomic structure of thymidylate synthase from Lactobacillus casei was determined at 3
angstrom resolution. The native enzyme is a dimer of identical subunits. The dimer interface …

5-Fluoro-2′-deoxyuridylate causes a rapid inactivation of thymidylate synthetase that is
dependent upon prior complexation of the cofactor 5, 10-methylenetetrahydrofolate. The …

Thymidylate synthetase forms a complex with 5-fluoro-2′-deoxyuridylate and 5, 10-
methylenetetrahydrofolate in which a nucleophile of the enzyme is covalently attached to the …

5-Fluorodeoxyuridine monophosphate (FdUMP), the active metabolite of 5-fluorouracil (5-
FU), is a tight-binding inhibitor of thymidylate synthetase, the enzyme which converts dUMP …

Structural modifications at the pyrimidine ring and at the C9, N10-bridge region of the
thymidylate synthase (TS) inhibitors N10-propargyl-5, 8-dideazafolate (1; PDDF; CB 3717) …

Department of Biochemistry and Biophysics, Department of Pharmaceutical Chemistry, and
Hormone Research Institute, University of California, San Francisco, San Francisco …

Leading articles type. However, some fem factors correspond to genes not identified before.
Of these, the femAB operon turned out to be particularly important, because (i) its …