Stepping on the gas to a circular economy: accelerating development of carbon-negative chemical production from gas fermentation
N Fackler, BD Heijstra, BJ Rasor… - Annual review of …, 2021 - annualreviews.org
Owing to rising levels of greenhouse gases in our atmosphere and oceans, climate change
poses significant environmental, economic, and social challenges globally. Technologies …
poses significant environmental, economic, and social challenges globally. Technologies …
The potential of CO2-based production cycles in biotechnology to fight the climate crisis
S Bachleitner, Ö Ata, D Mattanovich - Nature Communications, 2023 - nature.com
Rising CO2 emissions have pushed scientists to develop new technologies for a more
sustainable bio-based economy. Microbial conversion of CO2 and CO2-derived carbon …
sustainable bio-based economy. Microbial conversion of CO2 and CO2-derived carbon …
eQuilibrator 3.0: a database solution for thermodynamic constant estimation
ME Beber, MG Gollub, D Mozaffari… - Nucleic acids …, 2022 - academic.oup.com
Abstract eQuilibrator (equilibrator. weizmann. ac. il) is a database of biochemical equilibrium
constants and Gibbs free energies, originally designed as a web-based interface. While the …
constants and Gibbs free energies, originally designed as a web-based interface. While the …
Growth of E. coli on formate and methanol via the reductive glycine pathway
Engineering a biotechnological microorganism for growth on one-carbon intermediates,
produced from the abiotic activation of CO2, is a key synthetic biology step towards the …
produced from the abiotic activation of CO2, is a key synthetic biology step towards the …
High CO2 levels drive the TCA cycle backwards towards autotrophy
L Steffens, E Pettinato, TM Steiner, A Mall, S König… - Nature, 2021 - nature.com
It has recently been shown that in anaerobic microorganisms the tricarboxylic acid (TCA)
cycle, including the seemingly irreversible citrate synthase reaction, can be reversed and …
cycle, including the seemingly irreversible citrate synthase reaction, can be reversed and …
Four key steps control glycolytic flux in mammalian cells
Altered glycolysis is a hallmark of diseases including diabetes and cancer. Despite intensive
study of the contributions of individual glycolytic enzymes, systems-level analyses of flux …
study of the contributions of individual glycolytic enzymes, systems-level analyses of flux …
A new-to-nature carboxylation module to improve natural and synthetic CO2 fixation
M Scheffen, DG Marchal, T Beneyton, SK Schuller… - Nature Catalysis, 2021 - nature.com
The capture of CO2 by carboxylases is key to sustainable biocatalysis and a carbon-neutral
bio-economy, yet currently limited to few naturally existing enzymes. Here, we developed …
bio-economy, yet currently limited to few naturally existing enzymes. Here, we developed …
Construction and modular implementation of the THETA cycle for synthetic CO2 fixation
Synthetic biology offers the opportunity to build solutions for improved capture and
conversion of carbon dioxide (CO2) that outcompete those evolved by nature. Here we …
conversion of carbon dioxide (CO2) that outcompete those evolved by nature. Here we …
Self assembling nanoparticle enzyme clusters provide access to substrate channeling in multienzymatic cascades
Access to efficient enzymatic channeling is desired for improving all manner of designer
biocatalysis. We demonstrate that enzymes constituting a multistep cascade can self …
biocatalysis. We demonstrate that enzymes constituting a multistep cascade can self …
Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design
Acetyl-CoA is a fundamental metabolite for all life on Earth, and is also a key starting point
for the biosynthesis of a variety of industrial chemicals and natural products. Here we design …
for the biosynthesis of a variety of industrial chemicals and natural products. Here we design …