The advent of ribosome profiling and other tools to probe mRNA translation has revealed
that codon bias—the uneven use of synonymous codons in the transcriptome—serves as a …

This review summarizes our current understanding of translation in prokaryotes, focusing on
the mechanistic and structural aspects of each phase of translation: initiation, elongation …

No-go decay (NGD) is a eukaryotic quality control mechanism that evolved to cope with
translational arrests. The process is characterized by an endonucleolytic cleavage near the …

Comparative analyses of natural and mutated sequences have been used to probe
mechanisms of gene expression, but small sample sizes may produce biased outcomes. We …

Heterologous gene expression can be a significant burden for cells. Here we describe an in
vivo monitor that tracks changes in the capacity of Escherichia coli in real time and can be …

Deep sequencing now provides detailed snapshots of ribosome occupancy on mRNAs. We
leverage these data to parameterize a computational model of translation, keeping track of …

Synonymous codon changes, which do not alter protein sequence, were previously thought
to have no functional consequence. Although this concept has been overturned in recent …

Marina V. Rodnina is the winner of the 2015 Hans Neurath Award. Abstract The cellular
proteome is shaped by the combined activities of the gene expression and quality control …

The assembly of DNA from small fragments into large constructs has seen significant recent
development, becoming a pivotal technology in the ability to implement the vision of …

Protein homeostasis of bacterial cells is maintained by coordinated processes of protein
production, folding, and degradation. Translational efficiency of a given mRNA depends on …