Escherichia coli is the most preferred microorganism to express heterologous proteins for
therapeutic use, as around 30% of the approved therapeutic proteins are currently being …

Escherichia coli is one of the most widely used hosts for the production of heterologous
proteins and its genetics are far better characterized than those of any other microorganism …

Base editors use DNA-modifying enzymes targeted with a catalytically impaired CRISPR
protein to precisely install point mutations. Here, we develop phage-assisted continuous …

INTRODUCTION The stable recording of cellular events has the potential to advance our
understanding of a cell's history and how cells respond to stimuli. However, the construction …

Progress in our understanding of several biological processes promises to broaden the
usefulness of Escherichia coli as a tool for gene expression. There is an expanding choice …

HipA of Escherichia coli is a eukaryote-like serine-threonine kinase that inhibits cell growth
and induces persistence (multidrug tolerance). Previously, it was proposed that HipA inhibits …

Preparations enriched by a specific protein are rarely easily obtained from natural host cells.
Hence, recombinant protein production is frequently the sole applicable procedure. The …

The mechanisms whereby ribosomes engage a messenger RNA and select the start site for
translation differ between prokaryotes and eukaryotes. Initiation sites in polycistronic …

Valuable information on translation initiation is available from biochemical data and recently
solved structures. We present a detailed description of current knowledge about the …

One challenge for synthetic biologists is the predictable tuning of genetic circuit regulatory
components to elicit desired outputs. Gene expression driven by ligand-inducible …