1. Introduction 3082. Electron microscopy 3112.1 Specimen preparation 3112.2 The
electron microscope 3112.3 Acceleration voltage, defocus, and the electron gun 3122.4 …

In this review we present a broad survey of fundamental scientific and medically applied
studies on keyhole limpet hemocyanin (KLH). Commencing with the biochemistry of KLH …

Super-resolution microscopy opened diverse new avenues of research by overcoming the
resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual …

Resolution in optical nanoscopy (or super-resolution microscopy) depends on the
localization uncertainty and density of single fluorescent labels and on the sample's spatial …

A computational procedure is described for assigning the absolute hand of the structure of a
protein or assembly determined by single-particle electron microscopy. The procedure …

The resolution value claimed for an electron microscopical three-dimensional reconstruction
indicates the overall quality of the experiment. The Fourier shell correlation (FSC) criterion …

Cryoelectron microscopy of biological molecules is among the hottest growth areas in
biophysics and structural biology at present, and Frank is arguably the most distinguished …

Compartmentalization is an important organizational feature of life. It occurs at varying levels
of complexity ranging from eukaryotic organelles and the bacterial microcompartments, to …

Initiation of protein synthesis in eukaryotes requires recruitment of the 40 S ribosomal
subunit to the messenger RNA (mRNA). In most cases, this depends on recognition of a …

The mitochondrial ribosome is responsible for the biosynthesis of protein components
crucial to the generation of ATP in the eukaryotic cell. Because the protein: RNA ratio in the …