N-glycosylation design and control of therapeutic monoclonal antibodies
The N-linked glycan profiles on recombinant monoclonal antibody therapeutics significantly
affect antibody biological functions and are largely determined by host cell genotypes and …
affect antibody biological functions and are largely determined by host cell genotypes and …
Transient ammonia stress on Chinese hamster ovary (CHO) cells yield alterations to alanine metabolism and IgG glycosylation profiles
BF Synoground, CE McGraw, KS Elliott… - Biotechnology …, 2021 - Wiley Online Library
Background Ammonia concentrations typically increase during mammalian cell cultures,
mainly due to glutamine and other amino acid consumption. An early ammonia stress …
mainly due to glutamine and other amino acid consumption. An early ammonia stress …
Metabolic flux control in glycosylation
AG McDonald, JM Hayes, GP Davey - Current opinion in structural biology, 2016 - Elsevier
Highlights•Cellular metabolism influences glycosylation.•Flux through glycosyltransferase
networks is controlled by competition kinetics.•Nascent protein glycosylation is tailored …
networks is controlled by competition kinetics.•Nascent protein glycosylation is tailored …
Characterization of metabolic responses, genetic variations, and microsatellite instability in ammonia-stressed CHO cells grown in fed-batch cultures
DG Chitwood, Q Wang, K Elliott, A Bullock… - BMC …, 2021 - Springer
Background As bioprocess intensification has increased over the last 30 years, yields from
mammalian cell processes have increased from 10's of milligrams to over 10's of grams per …
mammalian cell processes have increased from 10's of milligrams to over 10's of grams per …
Assesment of Structure, Function, and Microevolutionary Dynamics of Extrachromosomal Circular DNA in Chinese Hamster Ovary Cells
D Chitwood - 2023 - tigerprints.clemson.edu
Chinese hamster ovary (CHO) cell lines are among the most popular expression hosts used
in biopharmaceutical manufacturing due to relative ease of culture, capacity to perform …
in biopharmaceutical manufacturing due to relative ease of culture, capacity to perform …
Animal cell expression systems
The glycan profile of therapeutic recombinant proteins such as monoclonal antibodies is a
critical quality attribute, which affects the efficacy of the final product. The cellular …
critical quality attribute, which affects the efficacy of the final product. The cellular …
[PDF][PDF] Enhanced understanding of protein glycosylation in CHO cells through computational tools and experimentation
P Kotidis - 2021 - core.ac.uk
Chinese hamster ovary (CHO) cells are the workhorse of the multibillion-dollar
biopharmaceuticals industry. They have been extensively harnessed for recombinant …
biopharmaceuticals industry. They have been extensively harnessed for recombinant …
Real-Time Monitoring of Cell Death Progress Using Capacitance Spectroscopy
S Wu - 2021 - search.proquest.com
Biologics, including the monoclonal antibody (mAb), has experienced rapid development in
the last decade. However, the price of biologics is often prohibitively high because of the low …
the last decade. However, the price of biologics is often prohibitively high because of the low …
Microsatellite instability as a tool to diagnose genome instability in CHO cell culture
DG Chitwood, Q Wang, K Elliott, A Bullock, D Jordana… - bioRxiv, 2019 - biorxiv.org
As bioprocess intensification has increased over the last 30 years, yields from mammalian
cell processes have increased from 10's of milligrams to over 10's of grams per liter. Most of …
cell processes have increased from 10's of milligrams to over 10's of grams per liter. Most of …
Method for reducing ammonium and lactate production in CHO cells
LEY Daniel, MR Andersen, HF Kildegaard - US Patent 10,961,556, 2021 - Google Patents
Ji and Obata,“Development of the GABA system in organotypic culture of hippocampal and
cerebellar slices from a 67-kDa isoform of glutamic acid decarboxylase (GAD67)-deficient …
cerebellar slices from a 67-kDa isoform of glutamic acid decarboxylase (GAD67)-deficient …