The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …

In the realm of biological research, the invention of super-resolution microscopy (SRM) has
enabled the visualization of ultrafine sub-cellular structures and their functions in live cells at …

Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Capturing mitochondria's intricate and dynamic structure poses a daunting challenge for
optical nanoscopy. Different labeling strategies have been demonstrated for live-cell …

Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …

Fluorescence resonance energy transfer (FRET) is a powerful spectroscopic method for
measuring distances in the 2–8 nm range. Often, conformational changes and molecular …

Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

Modern fluorescence-imaging methods promise to unveil organelle dynamics in live cells.
Phototoxicity, however, has become a prevailing issue when boosted illumination applies …

Maleimide-cysteine chemistry has been a routine practice for the site-specific labeling of
fluorophores to proteins since the 1950s. This approach, however, cannot bring out the best …

Photobleaching is the permanent loss of fluorescence after extended exposure to light and
is a major limiting factor in super‐resolution microscopy (SRM) that restricts spatiotemporal …