Technological advances in super-resolution microscopy to study cellular processes

C Bond, AN Santiago-Ruiz, Q Tang, M Lakadamyali - Molecular Cell, 2022 - cell.com
Since its initial demonstration in 2000, far-field super-resolution light microscopy has
undergone tremendous technological developments. In parallel, these developments have …

An introduction to optical super-resolution microscopy for the adventurous biologist

J Vangindertael, R Camacho, W Sempels… - … and applications in …, 2018 - iopscience.iop.org
Ever since the inception of light microscopy, the laws of physics have seemingly thwarted
every attempt to visualize the processes of life at its most fundamental, sub-cellular, level …

Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution

GCH Mo, B Ross, F Hertel, P Manna, X Yang… - Nature …, 2017 - nature.com
Compartmentalized biochemical activities are essential to all cellular processes, but there is
no generalizable method to visualize dynamic protein activities in living cells at a resolution …

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

H Deschout, T Lukes, A Sharipov, D Szlag… - Nature …, 2016 - nature.com
Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which
remains a challenge for super-resolution microscopy. Here we address this important issue …

Spectral cross-cumulants for multicolor super-resolved SOFI imaging

KS Grußmayer, S Geissbuehler, A Descloux… - Nature …, 2020 - nature.com
Super-resolution optical fluctuation imaging provides a resolution beyond the diffraction limit
by analysing stochastic fluorescence fluctuations with higher-order statistics. Using n th …

Embracing the uncertainty: the evolution of SOFI into a diverse family of fluctuation-based super-resolution microscopy methods

M Pawlowska, R Tenne, B Ghosh… - Journal of Physics …, 2021 - iopscience.iop.org
Super-resolution microscopy techniques have pushed-down the limits of resolution in optical
imaging by more than an order of magnitude. However, these methods often require long …

SOFIevaluator: a strategy for the quantitative quality assessment of SOFI data

B Moeyaert, W Vandenberg… - Biomedical Optics Express, 2020 - opg.optica.org
Super-resolution fluorescence imaging techniques allow optical imaging of specimens
beyond the diffraction limit of light. Super-resolution optical fluctuation imaging (SOFI) relies …

Semiconducting polymer dots with modulated photoblinking for high‐order super‐resolution optical fluctuation imaging

Z Sun, Z Liu, H Chen, R Li, Y Sun… - Advanced Optical …, 2019 - Wiley Online Library
Super‐resolution optical fluctuation imaging (SOFI) uses photoblinking to achieve fast,
background free, 3D fluorescence nanoscopy. The on/off photoblinking characteristics are …

Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI)

R Van den Eynde, A Sandmeyer… - Journal of Physics …, 2019 - iopscience.iop.org
Super-resolution (SR) fluorescence microscopy is typically carried out on research
microscopes equipped with high-NA TIRF objectives and powerful laser light sources. Super …

Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

S Duwé, W Vandenberg, P Dedecker - Chemical Communications, 2017 - pubs.rsc.org
We expand photochromic super-resolution optical fluctuation imaging (pcSOFI) to
monochromatic dual-channel sub-diffraction microscopy. Multi-tau (mt-) pcSOFI unmixes …