Electron-transfer mechanisms in amperometric biosensors

K Habermüller, M Mosbach, W Schuhmann - Fresenius' journal of …, 2000 - Springer
The function of amperometric biosensors is related to electron-transfer processes between
the active site of an (immobilized) enzyme and an electrode surface which is poised to an …

Amperometric lactate biosensors and their application in (sports) medicine, for life quality and wellbeing

N Nikolaus, B Strehlitz - Microchimica Acta, 2008 - Springer
The article reviews the use of electrochemical biosensors for detecting lactate, a key
metabolite of the anaerobic glycolytic pathway. This compound plays an important role in …

Glucose and lactate biosensors for scanning electrochemical microscopy imaging of single live cells

M Ciobanu, DE Taylor, JP Wilburn… - Analytical chemistry, 2008 - ACS Publications
We have developed glucose and lactate ultramicroelectrode (UME) biosensors based on
glucose oxidase and lactate oxidase (with enzymes immobilized onto Pt UMEs by either …

An improved amperometric L-lactate biosensor based on covalent immobilization of microbial lactate oxidase onto carboxylated multiwalled carbon nanotubes/copper …

K Dagar, CS Pundir - Enzyme and microbial technology, 2017 - Elsevier
An improved amperometric l-lactate biosensor was constructed based on covalent
immobilization of lactate oxidase (LOx) from Pediococcus species onto carboxylated …

In vivo voltammetric detection of rat brain lactate with carbon fiber microelectrodes coated with lactate oxidase

NF Shram, LI Netchiporouk, C Martelet… - Analytical …, 1998 - ACS Publications
To allow rat brain lactate measurement in vivo, a specific sensor based on a carbon fiber
(ϕ= 30 μm) microelectrode coated with lactate oxidase was prepared. Combined with the …

Evaluating the possibility of translating technological advances in non-invasive continuous lactate monitoring into critical care

RD Crapnell, A Tridente, CE Banks… - Sensors, 2021 - mdpi.com
Lactate is widely measured in critically ill patients as a robust indicator of patient
deterioration and response to treatment. Plasma concentrations represent a balance …

A bienzyme-poly-(o-phenylenediamine)-modified carbon paste electrode for the amperometric detection of L-lactate

MJ Lobo-Castanon, AJ Miranda-Ordieres… - Analytica Chimica …, 1997 - Elsevier
A reagentless lactate amperometric biosensor, constructed by immobilising the enzymes
glutamic pyruvic transaminase (GPT) and l-lactate dehydrogenase (LDH) together with its …

Directed evolution by accumulating tailored mutations: thermostabilization of lactate oxidase with less trade-off with catalytic activity

N Hamamatsu, Y Nomiya, T Aita… - Protein Engineering …, 2006 - academic.oup.com
We assumed that adverse effects posed by introducing multiple mutations could be
decomposed into those of each of the component mutations and that the risk could be …

Development of long life lactate sensor using thermostable mutant lactate oxidase

H Minagawa, N Nakayama, T Matsumoto… - Biosensors and …, 1998 - Elsevier
We examined a long life lactate sensor that employs two types of thermostable mutant
lactate oxidase, both generated by random mutagenesis. One of the mutants, LOD15 …

Immobilization enzyme fluorescence capillary analysis for determination of lactic acid

YS Li, X Ju, XF Gao, YY Zhao, YF Wu - Analytica chimica acta, 2008 - Elsevier
A new method for the determination of lactic acid based on the immobilization enzyme
fluorescence capillary analysis (IE-FCA) was proposed. Lactic dehydrogenase (LDH) was …