A decade of advances in transposon-insertion sequencing
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …
methods, which combine genome-wide transposon mutagenesis with high-throughput …
Best practices on the differential expression analysis of multi-species RNA-seq
Advances in transcriptome sequencing allow for simultaneous interrogation of differentially
expressed genes from multiple species originating from a single RNA sample, termed dual …
expressed genes from multiple species originating from a single RNA sample, termed dual …
An in vivo atlas of host–pathogen transcriptomes during Streptococcus pneumoniae colonization and disease
Streptococcus pneumoniae (Spn) colonizes the nasopharynx and can cause pneumonia.
From the lungs it spreads to the bloodstream and causes organ damage. We characterized …
From the lungs it spreads to the bloodstream and causes organ damage. We characterized …
Peptidyl-tRNA hydrolase is the nascent chain release factor in bacterial ribosome-associated quality control
Rescuing stalled ribosomes often involves their splitting into subunits. In many bacteria, the
resultant large subunits bearing peptidyl-tRNAs are processed by the ribosome-associated …
resultant large subunits bearing peptidyl-tRNAs are processed by the ribosome-associated …
An RNA-centric global view of Clostridioides difficile reveals broad activity of Hfq in a clinically important gram-positive bacterium
M Fuchs, V Lamm-Schmidt, J Sulzer… - Proceedings of the …, 2021 - National Acad Sciences
The gram-positive human pathogen Clostridioides difficile has emerged as the leading
cause of antibiotic-associated diarrhea. However, little is known about the bacterium's …
cause of antibiotic-associated diarrhea. However, little is known about the bacterium's …
Grad‐seq in a Gram‐positive bacterium reveals exonucleolytic sRNA activation in competence control
RNA–protein interactions are the crucial basis for many steps of bacterial gene expression,
including post‐transcriptional control by small regulatory RNA s (sRNA s). In stark contrast to …
including post‐transcriptional control by small regulatory RNA s (sRNA s). In stark contrast to …
[HTML][HTML] A computational system for identifying operons based on RNA-seq data
B Tjaden - Methods, 2020 - Elsevier
An operon is a set of neighboring genes in a genome that is transcribed as a single
polycistronic message. Genes that are part of the same operon often have related functional …
polycistronic message. Genes that are part of the same operon often have related functional …
Refining the pneumococcal competence regulon by RNA sequencing
J Slager, R Aprianto, JW Veening - Journal of bacteriology, 2019 - Am Soc Microbiol
Competence for genetic transformation allows the opportunistic human pathogen
Streptococcus pneumoniae to take up exogenous DNA for incorporation into its own …
Streptococcus pneumoniae to take up exogenous DNA for incorporation into its own …
Genome-scale transcription-translation mapping reveals features of Zymomonas mobilis transcription units and promoters
Zymomonas mobilis is an ethanologenic alphaproteobacterium with promise for the
industrial conversion of renewable plant biomass into fuels and chemical bioproducts …
industrial conversion of renewable plant biomass into fuels and chemical bioproducts …
Transcriptional organization and regulation of the Pseudomonas putida flagellar system
A Leal‐Morales, M Pulido‐Sánchez… - Environmental …, 2022 - Wiley Online Library
A single region of the Pseudomonas putida genome, designated the flagellar cluster,
includes 59 genes potentially involved in the biogenesis and function of the flagellar system …
includes 59 genes potentially involved in the biogenesis and function of the flagellar system …