Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …

Probing biological processes in living organisms that could provide one-of-a-kind insights
into real-time alterations of significant physiological parameters is a formidable task that …

Measurement of Förster resonance energy transfer by fluorescence lifetime imaging
microscopy (FLIM-FRET) is a powerful method for visualization of intracellular signaling …

The steady improvement in the imaging of cellular processes in living tissue over the last 10–
15 years through the use of various fluorophores including organic dyes, fluorescent …

Thermal green protein (TGP) is an extremely stable, highly soluble synthetic green
fluorescent protein. The quantum yield of TGP is lower than the closest related natural …

Fluorescence lifetime imaging microscopy (FLIM)-based Förster resonance energy transfer
(FRET) measurement (FLIM-FRET) is one of the powerful methods for imaging of …

Homogeneous proximity assays are widely implemented in high-throughput screening
(HTS) of small-molecule libraries for drug and probe discovery. Representative technologies …

In this article, we describe the engineering and X‐ray crystal structure of Thermal Green
Protein (TGP), an extremely stable, highly soluble, non‐aggregating green fluorescent …

We report the engineering of a new reversibly switching chromogenic protein, Dathail.
Dathail was evolved from the extremely thermostable fluorescent proteins thermal green …

Intracellular signal transduction involves a number of biochemical reactions, which largely
consist of protein-protein interactions and protein conformational changes. Monitoring …