Physics-based computational and theoretical approaches to intrinsically disordered proteins
Highlights•Liquid-liquid phase separation underlies the formation of membraneless
organelles within cells.•Theory and molecular simulations help to relate interactions at the …
organelles within cells.•Theory and molecular simulations help to relate interactions at the …
Principles governing the phase separation of multidomain proteins
A variety of membraneless organelles, often termed “biological condensates”, play an
important role in the regulation of cellular processes such as gene transcription, translation …
important role in the regulation of cellular processes such as gene transcription, translation …
Accurate model of liquid–liquid phase behavior of intrinsically disordered proteins from optimization of single-chain properties
Many intrinsically disordered proteins (IDPs) may undergo liquid–liquid phase separation
(LLPS) and participate in the formation of membraneless organelles in the cell, thereby …
(LLPS) and participate in the formation of membraneless organelles in the cell, thereby …
Disease‐linked TDP‐43 hyperphosphorylation suppresses TDP‐43 condensation and aggregation
LA Gruijs da Silva, F Simonetti, S Hutten… - The EMBO …, 2022 - embopress.org
Post‐translational modifications (PTMs) have emerged as key modulators of protein phase
separation and have been linked to protein aggregation in neurodegenerative disorders …
separation and have been linked to protein aggregation in neurodegenerative disorders …
Transmembrane coupling of liquid-like protein condensates
Liquid-liquid phase separation of proteins occurs on both surfaces of cellular membranes
during diverse physiological processes. In vitro reconstitution could provide insight into the …
during diverse physiological processes. In vitro reconstitution could provide insight into the …
Single-molecule FRET unmasks structural subpopulations and crucial molecular events during FUS low-complexity domain phase separation
Biomolecular condensates formed via phase separation of proteins and nucleic acids are
thought to be associated with a wide range of cellular functions and dysfunctions. We dissect …
thought to be associated with a wide range of cellular functions and dysfunctions. We dissect …
Improved coarse‐grained model for studying sequence dependent phase separation of disordered proteins
We present improvements to the hydropathy scale (HPS) coarse‐grained (CG) model for
simulating sequence‐specific behavior of intrinsically disordered proteins (IDPs), including …
simulating sequence‐specific behavior of intrinsically disordered proteins (IDPs), including …
Post-translational modifications in liquid-liquid phase separation: a comprehensive review
Liquid-liquid phase separation (LLPS) has received significant attention in recent biological
studies. It refers to a phenomenon that biomolecule exceeds the solubility, condensates and …
studies. It refers to a phenomenon that biomolecule exceeds the solubility, condensates and …
The protein folding problem: the role of theory
The protein folding problem was first articulated as question of how order arose from
disorder in proteins: How did the various native structures of proteins arise from interatomic …
disorder in proteins: How did the various native structures of proteins arise from interatomic …
Molecular details of protein condensates probed by microsecond long atomistic simulations
The formation of membraneless organelles in cells commonly occurs via liquid–liquid phase
separation (LLPS) and is in many cases driven by multivalent interactions between …
separation (LLPS) and is in many cases driven by multivalent interactions between …