[HTML][HTML] The ART of cryopreservation and its changing landscape

KO Pomeroy, P Comizzoli, JS Rushing, IL Lersten… - Fertility and …, 2022 - Elsevier
The purpose of this review is to educate the reader on the role that cryopreservation has
played and continues to play in the ever-evolving field of assisted reproductive technologies …

Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation

P Comizzoli, O Amelkina, PC Lee - Molecular Reproduction and …, 2022 - Wiley Online Library
Long‐term preservation of sperm, oocytes, and gonadal tissues at ambient temperatures
has the potential to lower the costs and simplify biobanking in human reproductive medicine …

Trehalose and alginate oligosaccharides enhance the stability of myofibrillar proteins in shrimp (Litopenaeus vannamei) muscle during frozen storage

J Chen, X Ying, S Deng, W Li… - Journal of Food …, 2022 - Wiley Online Library
Saccharin with antifreeze effects can reduce protein loss in some aquatic products and
effectively preserve taste and quality characteristics. However, the mechanisms responsible …

Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations

R Brogna, J Fan, H Sieme, WF Wolkers, H Oldenhof - Scientific reports, 2021 - nature.com
Even though dried sperm is not viable, it can be used for fertilization as long as its chromatin
remains intact. In this study, we investigated drying-and temperature-induced conformational …

Microwave-assisted dehydration, long-term storage at non-freezing temperatures, and rehydration of cat germinal vesicles

PC Lee, P Comizzoli - Biology of Reproduction, 2024 - academic.oup.com
Germinal vesicles are alternative targets for female fertility preservation due to their
availability and high resilience against non-physiological conditions. Preserved germinal …

Dried storage of sperm at supra-zero temperatures: An alternative for flow cytometric analysis under field conditions

J Rosero, AP dos Santos Silva, SCA Dos Santos… - Cryobiology, 2024 - Elsevier
In biotechnological processes such as chromosomal manipulation studies, semen has
become a reference in the ploidy verification of the evaluated material. However, the use of …

[HTML][HTML] The Effect of Choline Salt Addition to Trehalose Solution for Long-Term Storage of Dried and Viable Nuclei from Fully Grown Oocytes

JAO Cabral, PC Lee, S Wang, Y Wang, Y Zhang… - …, 2023 - ncbi.nlm.nih.gov
Although drying techniques are exciting alternatives to cryopreservation, it remains
challenging to maintain tightly controlled temperatures and humidity levels during storage of …

The Effect of Choline Salt Addition to Trehalose Solution for Long-Term Storage of Dried and Viable Nuclei from Fully Grown Oocytes

JA Orozco Cabral, PC Lee, S Wang, Y Wang, Y Zhang… - Bioengineering, 2023 - mdpi.com
Although drying techniques are exciting alternatives to cryopreservation, it remains
challenging to maintain tightly controlled temperatures and humidity levels during storage of …

Dry Preservation of Germinal Vesicles: Development of a New Formulation to Preserve DNA Integrity under Adverse Environmental Conditions

JAO Cabral - 2022 - search.proquest.com
Advances in the cryopreservation of genetic material has revolutionized human reproductive
medicine and animal conservation efforts. Despite many successes, oocytes have been …

Long term DNA storage evaluation and methodology

R Lehto - 2021 - knowledgecommons.lakeheadu.ca
DNA preservation over the long term is a considerable problem for many fields that still lack
a viable, cost-effective, long-term solution. The considerable amount of genetic material that …