Protein Engineering and High‐Throughput Screening by Yeast Surface Display: Survey of Current Methods

J Lopez-Morales, R Vanella, EA Appelt… - Small …, 2023 - Wiley Online Library
Yeast surface display (YSD) is a powerful tool in biotechnology that links genotype to
phenotype. In this review, the latest advancements in protein engineering and high …

Optimized single-cell gates for yeast display screening

X Pan, MO de Souza, FM Figueiras… - Protein Engineering …, 2025 - academic.oup.com
Yeast display is a widely used technology in antibody discovery and protein engineering.
The cell size of yeast enables fluorescence-activated cell sorting (FACS) to precisely screen …

Screening macrocyclic peptide libraries by yeast display allows control of selection process and affinity ranking

S Linciano, Y Mazzocato, Z Romanyuk, F Vascon… - bioRxiv, 2024 - biorxiv.org
Macrocyclic peptides provide an attractive modality for drug development due to their ability
to bind challenging targes, their small size, and amenability to powerful in vitro evolution …

Engineered yeast cells simulating CD19+ cancers to control CAR T cell activation

M Deichmann, G Schiesaro, K Ramanathan, K Zeeberg… - BioRxiv, 2023 - biorxiv.org
Chimeric antigen receptor (CAR) T cells have become an established form of
immunotherapy and continue to show promising results for the treatment of hematological …

Yeast-based screening platforms to understand and improve human health

M Deichmann, FG Hansson, ED Jensen - Trends in biotechnology, 2024 - cell.com
Detailed molecular understanding of the human organism is essential to develop effective
therapies. Saccharomyces cerevisiae has been used extensively for acquiring insights into …