Tools and techniques for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)/COVID-19 detection

SH Safiabadi Tali, JJ LeBlanc, Z Sadiq… - Clinical microbiology …, 2021 - Am Soc Microbiol
SUMMARY The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute
respiratory disease coronavirus 2 (SARS-CoV-2), has led to millions of confirmed cases and …

Recent developments on therapeutic and diagnostic approaches for COVID-19

J Majumder, T Minko - The AAPS journal, 2021 - Springer
The ongoing pandemic of coronavirus disease 2019 (COVID-19) caused by the severe
acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made a serious public health …

Assay techniques and test development for COVID-19 diagnosis

LJ Carter, LV Garner, JW Smoot, Y Li, Q Zhou… - 2020 - ACS Publications
An ongoing theme of the COVID-19 pandemic is the need for widespread availability of
accurate and efficient diagnostic testing for detection of SARS-CoV-2 and antiviral …

Coming of age: ten years of next-generation sequencing technologies

S Goodwin, JD McPherson, WR McCombie - Nature reviews genetics, 2016 - nature.com
Since the completion of the human genome project in 2003, extraordinary progress has
been made in genome sequencing technologies, which has led to a decreased cost per …

Schrödinger's microbes: tools for distinguishing the living from the dead in microbial ecosystems

JB Emerson, RI Adams, CMB Román, B Brooks… - Microbiome, 2017 - Springer
While often obvious for macroscopic organisms, determining whether a microbe is dead or
alive is fraught with complications. Fields such as microbial ecology, environmental health …

Microfluidics-based point-of-care testing (POCT) devices in dealing with waves of COVID-19 pandemic: the emerging solution

A Kumar, A Parihar, U Panda… - ACS applied bio …, 2022 - ACS Publications
Recent advances in microfluidics-based point-of-care testing (POCT) technology such as
paper, array, and beads have shown promising results for diagnosing various infectious …

The microglial sensome revealed by direct RNA sequencing

SE Hickman, ND Kingery, TK Ohsumi… - Nature …, 2013 - nature.com
Microglia, the principal neuroimmune sentinels of the brain, continuously sense changes in
their environment and respond to invading pathogens, toxins and cellular debris. Microglia …

A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequencing Quality Control Consortium

Nature biotechnology, 2014 - nature.com
We present primary results from the Sequencing Quality Control (SEQC) project,
coordinated by the US Food and Drug Administration. Examining Illumina HiSeq, Life …

Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction

J Ye, G Coulouris, I Zaretskaya, I Cutcutache… - BMC …, 2012 - Springer
Background Choosing appropriate primers is probably the single most important factor
affecting the polymerase chain reaction (PCR). Specific amplification of the intended target …

[HTML][HTML] Research techniques made simple: polymerase chain reaction (PCR)

L Garibyan, N Avashia - The Journal of investigative dermatology, 2013 - ncbi.nlm.nih.gov
The advent of the polymerase chain reaction (PCR) radically transformed biological science
from the time it was first discovered (Mullis, 1990). For the first time, it allowed for specific …