Nanopore sequencing of long ribosomal DNA amplicons enables portable and simple biodiversity assessments with high phylogenetic resolution across broad …

H Krehenwinkel, A Pomerantz, JB Henderson… - …, 2019 - academic.oup.com
Background In light of the current biodiversity crisis, DNA barcoding is developing into an
essential tool to quantify state shifts in global ecosystems. Current barcoding protocols often …

Interplay between developmental flexibility and determinism in the evolution of mimetic Heliconius wing patterns

C Concha, RWR Wallbank, JJ Hanly, J Fenner… - Current Biology, 2019 - cell.com
To what extent can we predict how evolution occurs? Do genetic architectures and
developmental processes canalize the evolution of similar outcomes in a predictable …

[HTML][HTML] A simple and efficient genomic DNA extraction protocol for large scale genetic analyses of plant biological systems

KC Sika, T Kefela, H Adoukonou-Sagbadja, L Ahoton… - Plant Gene, 2015 - Elsevier
Extraction of high quality genomic DNA from higher plants is hindered by the presence of
secondary metabolites, which reduce the yield and quality of the DNA. We describe an …

Molecular detection of 10 of the most unwanted alien forest pathogens in Canada using real-time PCR

J Lamarche, A Potvin, G Pelletier, D Stewart, N Feau… - PLoS …, 2015 - journals.plos.org
Invasive alien tree pathogens can cause significant economic losses as well as large-scale
damage to natural ecosystems. Early detection to prevent their establishment and spread is …

Trypanosomes infection, endosymbionts, and host preferences in tsetse flies (Glossina spp.) collected from Akagera park region, Rwanda: A correlational …

RS Gashururu, N Maingi, SM Githigia, DO Getange… - One Health, 2023 - Elsevier
Abstract Akagera National Park and its surroundings are home to tsetse flies and a number
of their mammalian hosts in Rwanda. A One-health approach is being used in the control …

[PDF][PDF] Protocol optimization for genomic DNA extraction and RAPD-PCR in mosquito larvae (Diptera: Culicidae)

S Gupta, S Preet - Annals of Biological Research, 2012 - academia.edu
ABSTRACT PCR based molecular markers are powerful tools for the analysis of genetic
diversity for which isolation of good quality genomic DNA is essential. This paper presents a …

Extraction of high-quality intact DNA from okra leaves despite their high

N Ahmed, S Nawaz, A Iqbal, M Mubin, A Butt… - Bioscience …, 2013 - bioscipublisher.com
The presence of mucilaginous acidic polysaccharides in okra leaves interferes with the
extraction of high-quality intact DNA for later restriction digestion and PCR amplification. We …

A plant cocktail amenable for PCR-based genetic analysis in Arabidopsis thaliana

SO Kotchoni, EW Gachomo… - Molecular biology reports, 2011 - Springer
Extraction of plant genomic DNA for subsequent genetic studies is often lengthy and difficult.
In addition, it requires the use of toxic reagents that remove secondary plant products, which …

Highly divergent Mollicutes symbionts coexist in the scorpion Androctonus australis

K Elmnasri, C Hamdi, B Ettoumi, E Crotti… - Journal of basic …, 2018 - Wiley Online Library
Androctonus australis is one of the most ubiquitous and common scorpion species in desert
and arid lands from North Africa to India and it has an important ecological role and social …

Dengue virus RNA purification from human plasma: a comparison of two techniques

RS Dettogni, ID Louro - Molecular biology reports, 2011 - Springer
Dengue virus RNA purification from human plasma is useful for research and clinical
purposes. Dengue is endemic in the Espirito Santo State, Brazil, and it is progressively …