Cells contain hundreds of organelles and macromolecular assemblies. Obtaining a
complete understanding of their intricate organization requires the nanometre-level, three …

It is becoming increasingly clear that mitochondria play an important role in neural function.
Recent studies show mitochondrial morphology to be crucial to cellular physiology and …

We describe a protocol for fully automated detection and segmentation of asymmetric,
presumed excitatory, synapses in serial electron microscopy images of the adult mammalian …

HIV-1-containing internal compartments are readily detected in images of thin sections from
infected cells using conventional transmission electron microscopy, but the origin …

For purposes of locating kerogen/organic matter and pores in SEM images of shale
samples, we tested an automated SEM-image segmentation workflow involving feature …

While there has been substantial progress in segmenting natural images, state-of-the-art
methods that perform well in such tasks unfortunately tend to underperform when confronted …

Understanding the hierarchical organization of molecules and organelles within the interior
of large eukaryotic cells is a challenge of fundamental interest in cell biology. We are using …

Electron microscopy (EM) facilitates analysis of the form, distribution, and functional status of
key organelle systems in various pathological processes, including those associated with …

Together, mitochondria and the endoplasmic reticulum (ER) occupy more than 20% of a
cell's volume, and morphological abnormality may lead to cellular function disorders. With …

High-resolution connectomics data allows for the identification of dysfunctional mitochondria
which are linked to a variety of diseases such as autism or bipolar. However, manual …