Despite the pre-eminence of the mouse in modelling human disease, several aspects of
murine biology limit its routine use in large-scale genetic and therapeutic screening. Many …

Brainbow is a genetic cell-labeling technique where hundreds of different hues can be
generated by stochastic and combinatorial expression of a few spectrally distinct fluorescent …

INTRODUCTION Organisms live by means of the complex, dynamic, three-dimensional (3D)
interplay between millions of components, from the molecular to the multicellular. Visualizing …

Vertebrates have greatly elaborated the basic chordate body plan and evolved highly
distinctive genomes that have been sculpted by two whole-genome duplications. Here we …

Transgenesis is an important tool for assessing gene function. In zebrafish, transgenesis has
suffered from three problems: the labor of building complex expression constructs using …

Advances in imaging and cell-labeling techniques have greatly enhanced our
understanding of developmental and neurobiological processes. Among vertebrates …

Targeted gene expression is a powerful approach to study the function of genes and cells in
vivo. In Drosophila, the P element-mediated Gal4-UAS method has been successfully used …

The Tol2 element is a naturally occurring active transposable element found in vertebrate
genomes. The Tol2 transposon system has been shown to be active from fish to mammals …

Despite the critical role of endothelial Wnt/β-catenin signaling during central nervous system
(CNS) vascularization, how endothelial cells sense and respond to specific Wnt ligands and …

Zebrafish provide a highly versatile model in which to study vertebrate development. Many
recent studies have elucidated early events in the organogenesis of the zebrafish pancreas; …